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TRNA methyltransferase

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<StructureSection load='1uak' size='350' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene=''>
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<StructureSection load='' size='350' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'>
== Function ==
== Function ==
'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.
'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.
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== Structural highlights ==
== Structural highlights ==
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Trm active site is located at the cleft at the subunit interface<ref>PMID:12773376</ref>.
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Trm <scene name='55/553109/Cv/4'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>. Water molecules are shown as red spheres.
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</StructureSection>
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==3D structures of tRNA methyltransferase==
==3D structures of tRNA methyltransferase==
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[[tRNA methyltransferase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*'''tRNA guanine 9 N1 methyltransferase'''
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**[[4jwg]] – fyTrm – fission yeast<br />
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**[[4jwf]], [[4jwh]] – fyTrm + SAH <br />
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**[[4jwj]] – yTrm + SAH – yeast<br />
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*'''tRNA guanine 37 N1 methyltransferase'''
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**[[3knu]] – ApTrm – ''Anaplasma phagocytophilum'' <br />
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**[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br />
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**[[3ief]] – Trm – ''Bartonella henselae'' <br />
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**[[1uaj]] – HiTrm – ''haemophilus influenzae''<br />
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**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />
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**[[3quv]] – Trm – ''Mycobacterium abscessus''<br />
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*Complexes of tRNA guanine 37 N1 methyltransferase
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**[[1uak]] – HiTrm + SAM <br />
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**[[1ual]], [[1uam]] – HiTrm + SAH <br />
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**[[3axz]] – HiTrm + adenosine<br />
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**[[4mcb]], [[4mcc]], [[4mcd]] – HiTrm + inhibitor<br />
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**[[1p9p]] – EcTrm + SAH – ''Escherichia coli'' <br />
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**[[2yx1]] – MjTrm + SFG – ''Methanocalcococcus jannaschii''<br />
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**[[3ay0]] – MjTrm + adenosine<br />
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**[[4h3y]], [[4h3z]] – Trm + SAH – ''Burkholderia phymatum'' <br />
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**[[4ig6]] – ApTrm + SAH <br />
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**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />
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*'''tRNA guanine 46 N7 methyltransferase'''
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**[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br />
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**[[2fca]] – Trm – ''Bacillus subtilis'' <br />
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**[[2vdu]], [[2vdv]] – yTrm <br />
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**[[3dxx]] – EcTrm <br />
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*Complexes of tRNA guanine 46 N7 methyltransferase
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**[[3ckk]] – Trm + SAM - human<br />
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**[[3dxy]] – EcTrm + SAM <br />
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**[[3dxz]] – EcTrm + SAH <br />
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*'''tRNA cytidine 34 O2 methyltransferase'''
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**[[3e5y]] – BpTrm – ''Burkholderia pseudomalllei'' <br />
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**[[4jak]], [[4kdz]] – EcTrm <br />
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*Complexes of tRNA cytidine 34 O2 methyltransferase
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**[[4kgn]] – BpTrm + SAH <br />
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**[[3n4k]], [[3n4j]] – Trm + SAH – ''Yersinia pestis'' <br />
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**[[1mxi]], [[1im8]] – HiTrm + SAH <br />
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**[[4jal]] – EcTrm + SAH<br />
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*'''tRNA guanine 6 N2 methyltransferase'''
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**[[3tm5]] – PfTrm + SFG – ''Pyrococcvus furiosus''<br />
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**[[3tlj]] – PfTrm + SAH<br />
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**[[3tm4]] – PfTrm + SAM<br />
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*'''tRNA adenine N1 methyltransferase'''
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**[[2pwy]] – Trm – ''Thermos thermophilus''<br />
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*'''tRNA uracil 54 methyltransferase'''
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**[[3bt7]] – yTrm + RNA<br />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry 1uak)

Drag the structure with the mouse to rotate

References

  1. ↑ Swinehart WE, Jackman JE. Diversity in mechanism and function of tRNA methyltransferases. RNA Biol. 2015;12(4):398-411. doi: 10.1080/15476286.2015.1008358. PMID:25626150 doi:http://dx.doi.org/10.1080/15476286.2015.1008358
  2. ↑ Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK. Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition. EMBO J. 2003 Jun 2;22(11):2593-603. PMID:12773376 doi:10.1093/emboj/cdg269

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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