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Pseudouridine synthase
From Proteopedia
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| + | <StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'> | ||
| + | __TOC__ | ||
| + | ==Function== | ||
| + | [[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction: | ||
| - | + | * [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule. | |
| - | + | ||
| - | + | * [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization. | |
== Structural highlights == | == Structural highlights == | ||
| - | The conversion of uridine to pseudouridine | + | The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]). |
| + | == 3D Structures of pseudouridine synthase == | ||
| + | [[Pseudouridine synthase 3D structures]] | ||
| + | |||
</StructureSection> | </StructureSection> | ||
| - | == 3D Structures of tRNA pseudouridine synthase== | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *tRNA pseudouridine synthase | ||
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| - | **[[1dj0]] – EcPUS I – ''Escherichia coli''<BR /> | ||
| - | **[[1ksk]], [[1ksl]] – EcPUS RsuA<BR /> | ||
| - | **[[1vio]] - PUS RsuA – ''Haemophilus influenzae''<BR /> | ||
| - | **[[1xpi]] - EcPUS RluC catalytic domain<BR /> | ||
| - | **[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <BR /> | ||
| - | **[[2ist]] - EcPUS RluD <BR /> | ||
| - | **[[1prz]], [[1qyu]], [[1v9f]] - EcPUS RluD catalytic domain<BR /> | ||
| - | **[[2olw]] - EcPUS RluE<BR /> | ||
| - | **[[2oml]] - EcPUS RluE catalytic domain<BR /> | ||
| - | **[[2gml]] - EcPUS RluF catalytic domain<BR /> | ||
| - | **[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human<br /> | ||
| - | **[[4nz7]] - hPUS Pus1 catalytic domain (mutant)<br /> | ||
| - | **[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR /> | ||
| - | **[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR /> | ||
| - | **[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD | ||
| - | |||
| - | *tRNA pseudouridine synthase complex with RNA | ||
| - | |||
| - | **[[1k8w]] – EcPUS B + stem-loop RNA<BR /> | ||
| - | **[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<BR /> | ||
| - | **[[2i82]] – EcPUS RluA + RNA<BR /> | ||
| - | **[[3dh3]] - EcPUS RluF + RNA<BR /> | ||
| - | **[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<BR /> | ||
| - | **[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<BR /> | ||
| - | **[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR /> | ||
| - | **[[3hjy]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA<BR /> | ||
| - | **[[1ze2]] - TmPUS B + RNA<BR /> | ||
| - | **[[2ab4]] - TmPUS B (mutant) + RNA<BR /> | ||
| - | **[[1r3e]], [[1r3f]] - EcPUS B + RNA<BR /> | ||
| - | *Probable tRNA pseudouridine synthase | ||
| - | **[[2apo]] – PPUS + ribosome biogenesis protein Nop10 – ''Methanocaldococcus jannaschii''<br /> | ||
| - | **[[2ey4]] - PfPPUS Cbf5 + ribosome biogenesis protein Nop10 + small nucleolar RNP <br /> | ||
| - | **[[2hvy]], [[3hax]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA<br /> | ||
| - | **[[2rfk]] - PfPUS B + ribosome biogenesis protein Nop10 + small nucleolar RNP + RNA<br /> | ||
| - | **[[3hay]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + small nucleolar RNP + RNA<br /> | ||
| - | **[[2aus]] – PPUS B + ribosome biogenesis protein Nop10 – ''Pyrococcus abyssi''<br /> | ||
| - | **[[1z2z]] – PPUS D – ''Methanosarcina mazei''<br /> | ||
| - | }} | ||
| - | == References == | ||
| - | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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