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| - | [[Image:1p25.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structure of nickel(II)-d(GGCGCC)2== |
| - | |PDB= 1p25 |SIZE=350|CAPTION= <scene name='initialview01'>1p25</scene>, resolution 2.90Å
| + | <StructureSection load='1p25' size='340' side='right'caption='[[1p25]], [[Resolution|resolution]] 2.90Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
| + | <table><tr><td colspan='2'>[[1p25]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P25 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p25 OCA], [https://pdbe.org/1p25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p25 RCSB], [https://www.ebi.ac.uk/pdbsum/1p25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p25 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[1p24|1P24]], [[1p26|1P26]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p25 OCA], [http://www.ebi.ac.uk/pdbsum/1p25 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p25 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Crystal structure of nickel(II)-d(GGCGCC)2'''
| + | [[Category: Delbaere LT]] |
| - | | + | [[Category: Labiuk SL]] |
| - | | + | [[Category: Lee JS]] |
| - | ==Overview==
| + | |
| - | Three novel X-ray crystal structures for the DNA hexamer d(GGCGCC) in the B-form complexed to divalent cobalt, nickel and zinc ions have been determined to a resolution of 2.9-3.0 A. The structures were isomorphous and had five DNA strands and five metal cations per asymmetric unit. In all three cases, divalent metal cations were coordinated only to the terminal guanine residue at the N(7) position, with no metal ions binding to non-terminal guanine positions. Water molecules bound to the metal cations interacted with neighboring guanine residues 3' to the ones to which the cations were coordinated, affecting the propeller twist. Even though DNA occupied only about 35% of the unit cell volume, it is interesting that the few interactions involving the metal cations were sufficient to stabilize the crystal lattice. As well as lending support to the proposal that these metals do not coordinate to B-DNA in a stable manner, the results presented here also extend the crystallographic evidence for this phenomenon to the GGC and CGC sequences for all three metal cations.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1P25 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P25 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Cobalt(II), nickel(II) and zinc(II) do not bind to intra-helical N(7) guanine positions in the B-form crystal structure of d(GGCGCC)., Labiuk SL, Delbaere LT, Lee JS, J Biol Inorg Chem. 2003 Sep;8(7):715-20. Epub 2003 Jul 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14505075 14505075]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Delbaere, L T.]] | + | |
| - | [[Category: Labiuk, S L.]] | + | |
| - | [[Category: Lee, J S.]] | + | |
| - | [[Category: b-dna]]
| + | |
| - | [[Category: nickel binding]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:53:44 2008''
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