1p5h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:05, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1p5h.jpg|left|200px]]
 
-
{{Structure
+
==Crystal structure of Formyl-CoA Transferase (apoenzyme) from Oxalobacter formigenes==
-
|PDB= 1p5h |SIZE=350|CAPTION= <scene name='initialview01'>1p5h</scene>, resolution 2.20&Aring;
+
<StructureSection load='1p5h' size='340' side='right'caption='[[1p5h]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1p5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oxalobacter_formigenes Oxalobacter formigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P5H FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
|GENE= FRC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=847 Oxalobacter formigenes])
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p5h OCA], [https://pdbe.org/1p5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p5h RCSB], [https://www.ebi.ac.uk/pdbsum/1p5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p5h ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=[[1p5r|1P5R]]
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p5h OCA], [http://www.ebi.ac.uk/pdbsum/1p5h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p5h RCSB]</span>
+
[https://www.uniprot.org/uniprot/FCTA_OXAFO FCTA_OXAFO] Catalyzes the transfer of the CoA moiety from formyl-CoA to oxalate. Essential enzyme for the bacterium survival, as it relies on oxalic acid as its sole source of energy.[HAMAP-Rule:MF_00742]
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p5/1p5h_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p5h ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal structure of Formyl-CoA Transferase (apoenzyme) from Oxalobacter formigenes'''
+
==See Also==
-
 
+
*[[Formyl-CoA transferase|Formyl-CoA transferase]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Formyl-CoA transferase catalyses transfer of CoA from formate to oxalate in the first step of oxalate degradation by Oxalobacter formigenes, a bacterium present in the intestinal flora which is implicated in oxalate catabolism in mammals. Formyl-CoA transferase is a member of a family of CoA-transferases for which no structural information is available. We now report the three-dimensional structure of O.formigenes formyl-CoA transferase, which reveals a novel fold and a very striking assembly of the homodimer. The subunit is composed of a large and a small domain where residues from both the N- and C-termini of the subunit are part of the large domain. The linkers between the domains give the subunit a circular shape with a hole in the middle. The enzyme monomers are tightly interacting and are interlocked. This fold requires drastic rearrangement of approximately 75 residues at the C-terminus for formation of the dimer. The structure of a complex of formyl-CoA transferase with CoA is also reported and sets the scene for a mechanistic understanding of enzymes of this family of CoA-transferases.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1P5H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oxalobacter_formigenes Oxalobacter formigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5H OCA].
+
-
 
+
-
==Reference==
+
-
Formyl-CoA transferase encloses the CoA binding site at the interface of an interlocked dimer., Ricagno S, Jonsson S, Richards N, Lindqvist Y, EMBO J. 2003 Jul 1;22(13):3210-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12839984 12839984]
+
[[Category: Oxalobacter formigenes]]
[[Category: Oxalobacter formigenes]]
-
[[Category: Single protein]]
+
[[Category: Jonsson S]]
-
[[Category: Jonsson, S.]]
+
[[Category: Lindqvist Y]]
-
[[Category: Lindqvist, Y.]]
+
[[Category: Ricagno S]]
-
[[Category: Ricagno, S.]]
+
[[Category: Richards N]]
-
[[Category: Richards, N.]]
+
-
[[Category: caib-baif family]]
+
-
[[Category: coa-transferase]]
+
-
[[Category: intertwined]]
+
-
[[Category: knotted fold]]
+
-
[[Category: oxalate]]
+
-
[[Category: oxalate degradation]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:55:08 2008''
+

Current revision

Crystal structure of Formyl-CoA Transferase (apoenzyme) from Oxalobacter formigenes

PDB ID 1p5h

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p5h"
Personal tools