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1par

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[[Image:1par.gif|left|200px]]
 
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{{Structure
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==DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE==
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|PDB= 1par |SIZE=350|CAPTION= <scene name='initialview01'>1par</scene>, resolution 2.600&Aring;
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<StructureSection load='1par' size='340' side='right'caption='[[1par]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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<table><tr><td colspan='2'>[[1par]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PAR FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1par FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1par OCA], [https://pdbe.org/1par PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1par RCSB], [https://www.ebi.ac.uk/pdbsum/1par PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1par ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1par FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1par OCA], [http://www.ebi.ac.uk/pdbsum/1par PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1par RCSB]</span>
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[https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
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}}
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__TOC__
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</StructureSection>
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'''DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE'''
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[[Category: Large Structures]]
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[[Category: Salmonella virus P22]]
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[[Category: Pabo CO]]
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==Overview==
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[[Category: Raumann BE]]
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Transcription of the ant gene during lytic growth of bacteriophage P22 (ref. 1) is regulated by the cooperative binding of two Arc repressor dimers to a 21-base-pair operator site. Here we report the co-crystal structure of this Arc tetramer-operator complex at 2.6 A resolution. As expected from genetic and structural studies and from the co-crystal structure of the homologous Escherichia coli MetJ repressor, each Arc dimer uses an antiparallel beta-sheet to recognize bases in the major groove. However, the Arc and MetJ complexes differ in several important ways: the beta-sheet-DNA interactions of Arc are far less symmetrical; DNA binding by Arc is accompanied by important conformational changes in the beta-sheet; and Arc uses a different part of its protein surface for dimer-dimer interactions.
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[[Category: Rould MA]]
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[[Category: Sauer RT]]
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==About this Structure==
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1PAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA].
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==Reference==
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DNA recognition by beta-sheets in the Arc repressor-operator crystal structure., Raumann BE, Rould MA, Pabo CO, Sauer RT, Nature. 1994 Feb 24;367(6465):754-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8107872 8107872]
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[[Category: Enterobacteria phage p22]]
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[[Category: Single protein]]
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[[Category: Pabo, C O.]]
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[[Category: Raumann, B E.]]
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[[Category: Rould, M A.]]
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[[Category: Sauer, R T.]]
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[[Category: double helix]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:57:15 2008''
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Current revision

DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE

PDB ID 1par

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