2cf4

From Proteopedia

(Difference between revisions)

Current revision

Pyrococcus horikoshii TET1 peptidase can assemble into a tetrahedron or a large octahedral shell

Structural highlights

2cf4 is a 1 chain structure with sequence from Pyrococcus horikoshii OT3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.08Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O58255_PYRHO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cellular proteolysis involves large oligomeric peptidases that play key roles in the regulation of many cellular processes. The cobalt-activated peptidase TET1 from the hyperthermophilic Archaea Pyrococcus horikoshii (PhTET1) was found to assemble as a 12-subunit tetrahedron and as a 24-subunit octahedral particle. Both quaternary structures were solved by combining x-ray crystallography and cryoelectron microscopy data. The internal organization of the PhTET1 particles reveals highly self-compartmentalized systems made of networks of access channels extended by vast catalytic chambers. The two edifices display aminopeptidase activity, and their organizations indicate substrate navigation mechanisms different from those described in other large peptidase complexes. Compared with the tetrahedron, the octahedron forms a more expanded hollow structure, representing a new type of giant peptidase complex. PhTET1 assembles into two different quaternary structures because of quasi-equivalent contacts that previously have only been identified in viral capsids.

An archaeal peptidase assembles into two different quaternary structures: A tetrahedron and a giant octahedron.,Schoehn G, Vellieux FM, Asuncion Dura M, Receveur-Brechot V, Fabry CM, Ruigrok RW, Ebel C, Roussel A, Franzetti B J Biol Chem. 2006 Nov 24;281(47):36327-37. Epub 2006 Sep 14. PMID:16973604^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schoehn G, Vellieux FM, Asuncion Dura M, Receveur-Brechot V, Fabry CM, Ruigrok RW, Ebel C, Roussel A, Franzetti B. An archaeal peptidase assembles into two different quaternary structures: A tetrahedron and a giant octahedron. J Biol Chem. 2006 Nov 24;281(47):36327-37. Epub 2006 Sep 14. PMID:16973604 doi:10.1074/jbc.M604417200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cf4"

@@ Line 1: / Line 1: @@
-==PYROCOCCUS HORIKOSHII TET1 PEPTIDASE CAN ASSEMBLE INTO A TETRAHEDRON OR A LARGE OCTAHEDRAL SHELL==
+==Pyrococcus horikoshii TET1 peptidase can assemble into a tetrahedron or a large octahedral shell==
-<StructureSection load='2cf4' size='340' side='right' caption='[[2cf4]], [[Resolution|resolution]] 3.08&Aring;' scene=''>
+<StructureSection load='2cf4' size='340' side='right'caption='[[2cf4]], [[Resolution|resolution]] 3.08&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2cf4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CF4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2cf4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CF4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.08&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cf4 OCA], [http://pdbe.org/2cf4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cf4 RCSB], [http://www.ebi.ac.uk/pdbsum/2cf4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cf4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cf4 OCA], [https://pdbe.org/2cf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cf4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cf4 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O58255_PYRHO O58255_PYRHO]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cf4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cf4_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 31: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Pyrococcus horikoshii]]
+[[Category: Large Structures]]
-[[Category: Dura, M A]]
+[[Category: Pyrococcus horikoshii OT3]]
-[[Category: Franzetti, B]]
+[[Category: Dura MA]]
-[[Category: Roussel, A]]
+[[Category: Franzetti B]]
-[[Category: Schoehn, G]]
+[[Category: Roussel A]]
-[[Category: Vellieux, F M.D]]
+[[Category: Schoehn G]]
-[[Category: Aminopeptidase]]
+[[Category: Vellieux FMD]]
-[[Category: Archaea]]
-[[Category: Hydrolase]]
-[[Category: Hyperthermophile]]
-[[Category: Metalloprotein]]
-[[Category: Tetrahedral]]

2cf4

From Proteopedia

Current revision

Pyrococcus horikoshii TET1 peptidase can assemble into a tetrahedron or a large octahedral shell

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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