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5ig9

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==Crystal structure of macrocyclase MdnC bound with precursor peptide MdnA from Microcystis aeruginosa MRC==
==Crystal structure of macrocyclase MdnC bound with precursor peptide MdnA from Microcystis aeruginosa MRC==
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<StructureSection load='5ig9' size='340' side='right' caption='[[5ig9]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
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<StructureSection load='5ig9' size='340' side='right'caption='[[5ig9]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5ig9]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IG9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5ig9]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_aeruginosa_MRC Microcystis aeruginosa MRC]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IG9 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ig8|5ig8]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.665&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ig9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ig9 OCA], [http://pdbe.org/5ig9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ig9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ig9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ig9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ig9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ig9 OCA], [https://pdbe.org/5ig9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ig9 RCSB], [https://www.ebi.ac.uk/pdbsum/5ig9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ig9 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B2G3D0_MICAE B2G3D0_MICAE]
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Macrocyclization is a common feature of natural product biosynthetic pathways including the diverse family of ribosomal peptides. Microviridins are architecturally complex cyanobacterial ribosomal peptides that target proteases with potent reversible inhibition. The product structure is constructed via three macrocyclizations catalyzed sequentially by two members of the ATP-grasp family, a unique strategy for ribosomal peptide macrocyclization. Here we describe in detail the structural basis for the enzyme-catalyzed macrocyclizations in the microviridin J pathway of Microcystis aeruginosa. The macrocyclases MdnC and MdnB interact with a conserved alpha-helix of the precursor peptide using a novel precursor-peptide recognition mechanism. The results provide insight into the unique protein-protein interactions that are key to the chemistry, suggest an origin for the natural combinatorial synthesis of microviridin peptides, and provide a framework for future engineering efforts to generate designed compounds.
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Structural basis for precursor protein-directed ribosomal peptide macrocyclization.,Li K, Condurso HL, Li G, Ding Y, Bruner SD Nat Chem Biol. 2016 Nov;12(11):973-979. doi: 10.1038/nchembio.2200. Epub 2016 Sep, 26. PMID:27669417<ref>PMID:27669417</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5ig9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bruner, S D]]
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[[Category: Large Structures]]
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[[Category: Condurso, H L]]
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[[Category: Microcystis aeruginosa MRC]]
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[[Category: Li, K]]
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[[Category: Bruner SD]]
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[[Category: Ligase]]
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[[Category: Condurso HL]]
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[[Category: Macrocyclase]]
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[[Category: Li K]]
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[[Category: Precursor peptide]]
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[[Category: Ripp]]
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Current revision

Crystal structure of macrocyclase MdnC bound with precursor peptide MdnA from Microcystis aeruginosa MRC

PDB ID 5ig9

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