1pey

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[[Image:1pey.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the Response Regulator Spo0F complexed with Mn2+==
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|PDB= 1pey |SIZE=350|CAPTION= <scene name='initialview01'>1pey</scene>, resolution 2.25&Aring;
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<StructureSection load='1pey' size='340' side='right'caption='[[1pey]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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<table><tr><td colspan='2'>[[1pey]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PEY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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|GENE= SPO0F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pey OCA], [https://pdbe.org/1pey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pey RCSB], [https://www.ebi.ac.uk/pdbsum/1pey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pey ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1nat|1NAT]], [[1srr|1SRR]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pey OCA], [http://www.ebi.ac.uk/pdbsum/1pey PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pey RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pe/1pey_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pey ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the Response Regulator Spo0F complexed with Mn2+'''
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==See Also==
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*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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As a part of studies on the structural characterization of the components of the sporulation phosphorelay in Bacillus subtilis, the crystal structure of the manganese derivative of an intermediate signal transducer, Spo0F, has been elucidated at 2.25 A resolution. The calcium complex and the apo structures have been analyzed previously. In apo Spo0F, the active-site cation cavity is only partially formed and it only becomes completed upon metal coordination. The carbonyl of Lys56 is coordinated to the metal and interestingly the side chain of Lys56 exists in a variety of conformations in the three crystal structures of Spo0F. The affinity of the magnesium ion for Spo0F is in fact low; however, it binds Spo0F when it is in complex with Spo0B. It is proposed that the existence of a deep pocket which extends from the surface to the metal site could attract and direct the metal, thereby facilitating the metal binding of the complex.
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==About this Structure==
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1PEY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PEY OCA].
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==Reference==
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Metals in the sporulation phosphorelay: manganese binding by the response regulator Spo0F., Mukhopadhyay D, Sen U, Zapf J, Varughese KI, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):638-45. Epub 2004, Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15039551 15039551]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Mukhopadhyay, D.]]
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[[Category: Mukhopadhyay D]]
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[[Category: Sen, U.]]
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[[Category: Sen U]]
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[[Category: Varughese, K I.]]
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[[Category: Varughese KI]]
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[[Category: Zapf, J.]]
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[[Category: Zapf J]]
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[[Category: (alpha/beta) protein]]
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[[Category: cation binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:58:45 2008''
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Current revision

Crystal structure of the Response Regulator Spo0F complexed with Mn2+

PDB ID 1pey

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