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1pfp

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[[Image:1pfp.jpg|left|200px]]
 
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{{Structure
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==CATHELIN-LIKE MOTIF OF PROTEGRIN-3==
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|PDB= 1pfp |SIZE=350|CAPTION= <scene name='initialview01'>1pfp</scene>, resolution 2.30&Aring;
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<StructureSection load='1pfp' size='340' side='right'caption='[[1pfp]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CSE:SELENOCYSTEINE'>CSE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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<table><tr><td colspan='2'>[[1pfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFP FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= NPG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfp OCA], [https://pdbe.org/1pfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfp RCSB], [https://www.ebi.ac.uk/pdbsum/1pfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfp ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1kwi|1KWI]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfp OCA], [http://www.ebi.ac.uk/pdbsum/1pfp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pfp RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PG3_PIG PG3_PIG] Microbicidal activity. Active against E.coli, Listeria monocytogenes and C.albicans, in vitro.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/1pfp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pfp ConSurf].
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<div style="clear:both"></div>
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'''CATHELIN-LIKE MOTIF OF PROTEGRIN-3'''
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==See Also==
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*[[Protegrin|Protegrin]]
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__TOC__
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==Overview==
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</StructureSection>
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A protocol for the quantitative incorporation of both selenomethionine and selenocysteine into recombinant proteins overexpressed in Escherichia coli is described. This methodology is based on the use of a suitable cysteine auxotrophic strain and a minimal medium supplemented with selenium-labeled methionine and cysteine. The proteins chosen for these studies are the cathelin-like motif of protegrin-3 and a nucleoside-diphosphate kinase. Analysis of the purified proteins by electrospray mass spectrometry and X-ray crystallography revealed that both cysteine and methionine residues were isomorphously replaced by selenocysteine and selenomethionine. Moreover, selenocysteines allowed the formation of unstrained and stable diselenide bridges in place of the canonical disulfide bonds. In addition, we showed that NDP kinase contains a selenocysteine adduct on Cys122. This novel selenium double-labeling method is proposed as a general approach to increase the efficiency of the MAD technique used for phase determination in protein crystallography.
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[[Category: Large Structures]]
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==About this Structure==
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1PFP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFP OCA].
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==Reference==
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Selenomethionine and selenocysteine double labeling strategy for crystallographic phasing., Strub MP, Hoh F, Sanchez JF, Strub JM, Bock A, Aumelas A, Dumas C, Structure. 2003 Nov;11(11):1359-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14604526 14604526]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Aumelas, A.]]
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[[Category: Aumelas A]]
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[[Category: Bock, A.]]
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[[Category: Bock A]]
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[[Category: Dumas, C.]]
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[[Category: Dumas C]]
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[[Category: Hoh, F.]]
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[[Category: Hoh F]]
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[[Category: Sanchez, J F.]]
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[[Category: Sanchez J-F]]
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[[Category: Strub, J M.]]
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[[Category: Strub JM]]
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[[Category: Strub, M P.]]
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[[Category: Strub M-P]]
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[[Category: diselenide]]
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[[Category: pg-3]]
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[[Category: secy]]
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[[Category: semet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:59:00 2008''
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Current revision

CATHELIN-LIKE MOTIF OF PROTEGRIN-3

PDB ID 1pfp

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