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| - | [[Image:1pjf.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage== |
| - | |PDB= 1pjf |SIZE=350|CAPTION= <scene name='initialview01'>1pjf</scene>
| + | <StructureSection load='1pjf' size='340' side='right'caption='[[1pjf]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[1pjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_Pf1 Pseudomonas virus Pf1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJF FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr> |
| - | |GENE= | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjf OCA], [https://pdbe.org/1pjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjf RCSB], [https://www.ebi.ac.uk/pdbsum/1pjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjf ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjf OCA], [http://www.ebi.ac.uk/pdbsum/1pjf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pjf RCSB]</span>
| + | [https://www.uniprot.org/uniprot/CAPSD_BPPF1 CAPSD_BPPF1] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity). |
| - | }}
| + | |
| | | | |
| - | '''Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage'''
| + | ==See Also== |
| - | | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | The atomic resolution structure of Pf1 coat protein determined by solid-state NMR spectroscopy of magnetically aligned filamentous bacteriophage particles in solution is compared to the structures previously determined by X-ray fiber and neutron diffraction, the structure of its membrane-bound form, and the structure of fd coat protein. These structural comparisons provide insights into several biological properties, differences between class I and class II filamentous bacteriophages, and the assembly process. The six N-terminal amino acid residues adopt an unusual "double hook" conformation on the outside of the bacteriophage particle. The solid-state NMR results indicate that at 30 degrees C, some of the coat protein subunits assume a single, fully structured conformation, and some have a few mobile residues that provide a break between two helical segments, in agreement with structural models from X-ray fiber and neutron diffraction, respectively. The atomic resolution structure determined by solid-state NMR for residues 7-14 and 18-46, which excludes the N-terminal double hook and the break between the helical segments, but encompasses more than 80% of the backbone including the distinct kink at residue 29, agrees with that determined by X-ray fiber diffraction with an RMSD value of 2.0 A. The symmetry and distance constraints determined by X-ray fiber and neutron diffraction enable the construction of an accurate model of the bacteriophage particle from the coordinates of the coat protein monomers.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Pseudomonas virus Pf1]] |
| - | ==About this Structure==
| + | [[Category: Nevzorov AA]] |
| - | 1PJF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA].
| + | [[Category: Opella SJ]] |
| - | | + | [[Category: Thiriot DS]] |
| - | ==Reference==
| + | [[Category: Wu CH]] |
| - | Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy., Thiriot DS, Nevzorov AA, Zagyanskiy L, Wu CH, Opella SJ, J Mol Biol. 2004 Aug 13;341(3):869-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15288792 15288792]
| + | [[Category: Zagyanskiy L]] |
| - | [[Category: Pseudomonas phage pf1]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Nevzorov, A A.]] | + | |
| - | [[Category: Opella, S J.]] | + | |
| - | [[Category: Thiriot, D S.]] | + | |
| - | [[Category: Wu, C H.]] | + | |
| - | [[Category: Zagyanskiy, L.]] | + | |
| - | [[Category: helical virus]]
| + | |
| - | [[Category: virus/viral protein]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:00:28 2008''
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