5c9l
From Proteopedia
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==Crystal structure of native PLL lectin from Photorhabdus luminescens at 1.65 A resolution== | ==Crystal structure of native PLL lectin from Photorhabdus luminescens at 1.65 A resolution== | ||
- | <StructureSection load='5c9l' size='340' side='right' caption='[[5c9l]], [[Resolution|resolution]] 1.65Å' scene=''> | + | <StructureSection load='5c9l' size='340' side='right'caption='[[5c9l]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>[[5c9l]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[5c9l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C9L FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9l OCA], [https://pdbe.org/5c9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c9l RCSB], [https://www.ebi.ac.uk/pdbsum/5c9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9l ProSAT]</span></td></tr> |
</table> | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/Q7N8J0_PHOLL Q7N8J0_PHOLL] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity towards insect larvae. A hypothetical protein from P. luminescens was identified, purified from the native source and characterized as an L-fucose-binding lectin, named PLL. Glycan array and biochemical characterization data revealed PLL to be specific towards L-fucose and the disaccharide glycan 3,6-O-Me2-Glcbeta1-4(2,3-O-Me2)Rhaalpha-O-(p-C6H4)-OCH2CH2NH2. PLL was discovered to be a homo-tetramer with an inter-subunit disulphide bridge. The crystal structures of native (ntPLL) and recombinant PLL (rPLL) revealed a seven-bladed beta-propeller fold creating 7 putative fucose-binding sites per monomer. The crystal structure of the rPLL/L-fucose complex confirmed at least three sites being fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C-terminus of the lectin into one of these sites. PLL exhibited a binding ability to insect haemocytes and the cuticular surface of a nematode, Heterorhabditis bacteriophora. | ||
+ | |||
+ | A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Hepta-Bladed beta-Propeller Tetrameric Structure.,Kumar A, Sykorova P, Demo G, Dobes P, Hyrsl P, Wimmerova M J Biol Chem. 2016 Oct 7. pii: jbc.M115.693473. PMID:27758853<ref>PMID:27758853</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 5c9l" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
+ | [[Category: Large Structures]] | ||
[[Category: Photorhabdus luminescens]] | [[Category: Photorhabdus luminescens]] | ||
- | [[Category: Demo | + | [[Category: Demo G]] |
- | [[Category: Dobes | + | [[Category: Dobes P]] |
- | [[Category: Hyrsl | + | [[Category: Hyrsl P]] |
- | [[Category: Kumar | + | [[Category: Kumar A]] |
- | [[Category: Sykorova | + | [[Category: Sykorova P]] |
- | [[Category: Wimmerova | + | [[Category: Wimmerova M]] |
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Current revision
Crystal structure of native PLL lectin from Photorhabdus luminescens at 1.65 A resolution
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