5h2k
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5h2k is ON HOLD Authors: Royant, A., Nango, E., Nakane, T., Tanaka, T., Arima, T., Neutze, R., Iwata, S. Description: A three dimensional movie of ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==A three dimensional movie of structural changes in bacteriorhodopsin: structure obtained 2 us after photoexcitation== | |
| + | <StructureSection load='5h2k' size='340' side='right'caption='[[5h2k]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5h2k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H2K FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C14:TETRADECANE'>C14</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=DD9:NONANE'>DD9</scene>, <scene name='pdbligand=HP6:HEPTANE'>HP6</scene>, <scene name='pdbligand=L2P:2,3-DI-PHYTANYL-GLYCEROL'>L2P</scene>, <scene name='pdbligand=MYS:PENTADECANE'>MYS</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene>, <scene name='pdbligand=TRD:TRIDECANE'>TRD</scene>, <scene name='pdbligand=UND:UNDECANE'>UND</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h2k OCA], [https://pdbe.org/5h2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h2k RCSB], [https://www.ebi.ac.uk/pdbsum/5h2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h2k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient. | ||
| - | + | A three-dimensional movie of structural changes in bacteriorhodopsin.,Nango E, Royant A, Kubo M, Nakane T, Wickstrand C, Kimura T, Tanaka T, Tono K, Song C, Tanaka R, Arima T, Yamashita A, Kobayashi J, Hosaka T, Mizohata E, Nogly P, Sugahara M, Nam D, Nomura T, Shimamura T, Im D, Fujiwara T, Yamanaka Y, Jeon B, Nishizawa T, Oda K, Fukuda M, Andersson R, Bath P, Dods R, Davidsson J, Matsuoka S, Kawatake S, Murata M, Nureki O, Owada S, Kameshima T, Hatsui T, Joti Y, Schertler G, Yabashi M, Bondar AN, Standfuss J, Neutze R, Iwata S Science. 2016 Dec 23;354(6319):1552-1557. doi: 10.1126/science.aah3497. PMID:28008064<ref>PMID:28008064</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Arima | + | <div class="pdbe-citations 5h2k" style="background-color:#fffaf0;"></div> |
| - | [[Category: Iwata | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Halobacterium salinarum NRC-1]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Arima T]] | ||
| + | [[Category: Iwata S]] | ||
| + | [[Category: Nakane T]] | ||
| + | [[Category: Nango E]] | ||
| + | [[Category: Neutze R]] | ||
| + | [[Category: Royant A]] | ||
| + | [[Category: Tanaka T]] | ||
Current revision
A three dimensional movie of structural changes in bacteriorhodopsin: structure obtained 2 us after photoexcitation
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