5m10
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of cyclohexanone monooxygenase from Thermocrispum municipale in the oxidised state with a bound nicotinamide.== | |
| + | <StructureSection load='5m10' size='340' side='right'caption='[[5m10]], [[Resolution|resolution]] 1.22Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5m10]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermocrispum_municipale Thermocrispum municipale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M10 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.22Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m10 OCA], [https://pdbe.org/5m10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m10 RCSB], [https://www.ebi.ac.uk/pdbsum/5m10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m10 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1L1QK40_9PSEU A0A1L1QK40_9PSEU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cyclohexanone monooxygenase (CHMO) is a promising biocatalyst for industrial reactions owing to its broad substrate spectrum and excellent regio-, chemo-, and enantioselectivity. However, the low stability of many Baeyer-Villiger monooxygenases is an obstacle for their exploitation in industry. Characterization and crystal structure determination of a robust CHMO from Thermocrispum municipale is reported. The enzyme efficiently converts a variety of aliphatic, aromatic, and cyclic ketones, as well as prochiral sulfides. A compact substrate-binding cavity explains its preference for small rather than bulky substrates. Small-scale conversions with either purified enzyme or whole cells demonstrated the remarkable properties of this newly discovered CHMO. The exceptional solvent tolerance and thermostability make the enzyme very attractive for biotechnology. | ||
| - | + | Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase.,Romero E, Castellanos JR, Mattevi A, Fraaije MW Angew Chem Int Ed Engl. 2016 Nov 22. doi: 10.1002/anie.201608951. PMID:27873437<ref>PMID:27873437</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Gomez-Castellanos | + | <div class="pdbe-citations 5m10" style="background-color:#fffaf0;"></div> |
| - | [[Category: Mattevi | + | |
| + | ==See Also== | ||
| + | *[[Monooxygenase 3D structures|Monooxygenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermocrispum municipale]] | ||
| + | [[Category: Gomez-Castellanos JR]] | ||
| + | [[Category: Mattevi A]] | ||
Current revision
Crystal structure of cyclohexanone monooxygenase from Thermocrispum municipale in the oxidised state with a bound nicotinamide.
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