1pp5

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[[Image:1pp5.gif|left|200px]]
 
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{{Structure
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==Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot==
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|PDB= 1pp5 |SIZE=350|CAPTION= <scene name='initialview01'>1pp5</scene>
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<StructureSection load='1pp5' size='340' side='right'caption='[[1pp5]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1pp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PP5 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
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|GENE= MCJA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PRD_000184:Microcin+J25'>PRD_000184</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pp5 OCA], [https://pdbe.org/1pp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pp5 RCSB], [https://www.ebi.ac.uk/pdbsum/1pp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pp5 ProSAT], [https://www.topsan.org/Proteins/NESGC/1pp5 TOPSAN]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pp5 OCA], [http://www.ebi.ac.uk/pdbsum/1pp5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pp5 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MCJA_ECOLX MCJA_ECOLX] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.<ref>PMID:11731133</ref> <ref>PMID:11443089</ref> <ref>PMID:12401787</ref>
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<div style="background-color:#fffaf0;">
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'''Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot'''
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== Publication Abstract from PubMed ==
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==Overview==
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The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.
The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.
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==About this Structure==
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Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot.,Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH J Am Chem Soc. 2003 Oct 15;125(41):12382-3. PMID:14531661<ref>PMID:14531661</ref>
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1PP5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PP5 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot., Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH, J Am Chem Soc. 2003 Oct 15;125(41):12382-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14531661 14531661]
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</div>
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<div class="pdbe-citations 1pp5" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bayro, M J.]]
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[[Category: Bayro MJ]]
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[[Category: Ebright, R H.]]
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[[Category: Ebright RH]]
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[[Category: Huang, J Y.]]
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[[Category: Huang JY]]
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[[Category: Ma, L C.]]
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[[Category: Ma L-C]]
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[[Category: Montelione, G T.]]
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[[Category: Montelione GT]]
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[[Category: Mukhopadhyay, J.]]
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[[Category: Mukhopadhyay J]]
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[[Category: NESG, Northeast Structural Genomics Consortium.]]
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[[Category: Swapna GVT]]
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[[Category: Swapna, G V.T.]]
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[[Category: backbone-sidechain amide linkage]]
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[[Category: lariat]]
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[[Category: nesg]]
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[[Category: northeast structural genomics consortium]]
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[[Category: protein structure initiative]]
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[[Category: protoknot]]
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[[Category: psi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:02:38 2008''
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Current revision

Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot

PDB ID 1pp5

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