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5f5q

From Proteopedia

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Current revision

Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside

Structural highlights

5f5q is a 2 chain structure with sequence from Canavalia cathartica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.52Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CONV_CANCT D-mannose/D-glucose-binding lectin which binds alpha-methyl-D-mannoside, D-mannose and D-glucose in that order (PubMed:24519628, PubMed:27737777). Also binds to serum fetuin and ovalbumin (PubMed:24519628). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:24519628). Is not toxic towards larvae of the brine shrimp Artemia (PubMed:24519628). Induces relaxation in rat endothelized aorta (PubMed:24519628). Shows a transient edematogenic effect in rat (PubMed:27737777).^[1] ^[2]

Publication Abstract from PubMed

A lectin from Canavalia virosa, Diocleinae subtribe, was purified by affinity chromatography with Sephadex G-50 matrix and named ConV. The primary structure of ConV was obtained by mass spectrometry and crystals were obtained by the vapor diffusion method at 293K and belonged to orthorhombic space group P21221 with two molecules in its asymmetric unit. The structure obtained presented Rfactor and Rfree of 18.91% and 24.92% respectively, with no residues in nonallowed regions of Ramachandran plot. The crystal structure was solved at 2.53A and was demonstrated to be very similar to other lectins from the same subtribe. In inflammatory tests, ConV elicited paw edema, but incubation of lectin with glucose beforehand was able to reduce the edematogenic effect, indicating the involvement of the carbohydrate recognition domain in this process. The lectin also showed toxicity to rat C6 glioma cells, disrupting the mitochondrial membrane potential (DeltaYm) and decreasing cell viability, indicating an anticancer potential for ConV. In silico studies confirmed that ConV interacts strongly with carbohydrates that comprise the N-glycans of glycoproteins. This finding corroborates the hypothesis which holds that the lectin domain interacts with glycans in molecular targets and that this contributes to the effects observed in biological activities.

Structural characterization of a lectin from Canavalia virosa seeds with inflammatory and cytotoxic activities.,Osterne VJ, Silva-Filho JC, Santiago MQ, Pinto-Junior VR, Almeida AC, Barreto AA, Wolin IA, Nascimento AP, Amorim RM, Rocha BA, Delatorre P, Nagano CS, Leal RB, Assreuy AM, Nascimento KS, Cavada BS Int J Biol Macromol. 2016 Oct 11;94(Pt A):271-282. doi:, 10.1016/j.ijbiomac.2016.10.020. PMID:27737777^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Osterne VJ, Santiago MQ, Pinto-Junior VR, Cajazeiras JB, Correia JL, Leitao CC, Carneiro RF, Pereira-Junior FN, Vasconcelos MA, Rocha BA, Assreuy AM, Bringel PH, Nagano CS, Nascimento KS, Cavada BS. Purification, partial characterization, and CNBr-sepharose immobilization of a vasorelaxant glucose/mannose lectin from Canavalia virosa seeds. Appl Biochem Biotechnol. 2014 Apr;172(7):3342-53. doi: 10.1007/s12010-014-0751-3., Epub 2014 Feb 13. PMID:24519628 doi:http://dx.doi.org/10.1007/s12010-014-0751-3
↑ Osterne VJ, Silva-Filho JC, Santiago MQ, Pinto-Junior VR, Almeida AC, Barreto AA, Wolin IA, Nascimento AP, Amorim RM, Rocha BA, Delatorre P, Nagano CS, Leal RB, Assreuy AM, Nascimento KS, Cavada BS. Structural characterization of a lectin from Canavalia virosa seeds with inflammatory and cytotoxic activities. Int J Biol Macromol. 2016 Oct 11;94(Pt A):271-282. doi:, 10.1016/j.ijbiomac.2016.10.020. PMID:27737777 doi:http://dx.doi.org/10.1016/j.ijbiomac.2016.10.020
↑ Osterne VJ, Silva-Filho JC, Santiago MQ, Pinto-Junior VR, Almeida AC, Barreto AA, Wolin IA, Nascimento AP, Amorim RM, Rocha BA, Delatorre P, Nagano CS, Leal RB, Assreuy AM, Nascimento KS, Cavada BS. Structural characterization of a lectin from Canavalia virosa seeds with inflammatory and cytotoxic activities. Int J Biol Macromol. 2016 Oct 11;94(Pt A):271-282. doi:, 10.1016/j.ijbiomac.2016.10.020. PMID:27737777 doi:http://dx.doi.org/10.1016/j.ijbiomac.2016.10.020

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5f5q"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside==
-<StructureSection load='5f5q' size='340' side='right' caption='[[5f5q]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
+<StructureSection load='5f5q' size='340' side='right'caption='[[5f5q]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5f5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F5Q FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5f5q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F5Q FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5q OCA], [http://pdbe.org/5f5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5q RCSB], [http://www.ebi.ac.uk/pdbsum/5f5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5q ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5q OCA], [https://pdbe.org/5f5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f5q RCSB], [https://www.ebi.ac.uk/pdbsum/5f5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5q ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CONA_CANCT CONA_CANCT]] Glucose/D-mannose specific lectin.
+[https://www.uniprot.org/uniprot/CONV_CANCT CONV_CANCT] D-mannose/D-glucose-binding lectin which binds alpha-methyl-D-mannoside, D-mannose and D-glucose in that order (PubMed:24519628, PubMed:27737777). Also binds to serum fetuin and ovalbumin (PubMed:24519628). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:24519628). Is not toxic towards larvae of the brine shrimp Artemia (PubMed:24519628). Induces relaxation in rat endothelized aorta (PubMed:24519628). Shows a transient edematogenic effect in rat (PubMed:27737777).<ref>PMID:24519628</ref> <ref>PMID:27737777</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5f5q" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Concanavalin 3D structures|Concanavalin 3D structures]]
 == References ==
 <references/>
@@ Line 23: / Line 27: @@
 </StructureSection>
 [[Category: Canavalia cathartica]]
-[[Category: Cavada, B S]]
+[[Category: Large Structures]]
-[[Category: Delatorre, P]]
+[[Category: Cavada BS]]
-[[Category: Lossio, C F]]
+[[Category: Delatorre P]]
-[[Category: Nascimento, K S]]
+[[Category: Lossio CF]]
-[[Category: Osterne, V J.S]]
+[[Category: Nascimento KS]]
-[[Category: Pinto-Junior, V R]]
+[[Category: Osterne VJS]]
-[[Category: Santiago, M Q]]
+[[Category: Pinto-Junior VR]]
-[[Category: Silva-Filho, J C]]
+[[Category: Santiago MQ]]
-[[Category: Metal binding protein]]
+[[Category: Silva-Filho JC]]
-[[Category: Sugar binding protein]]

5f5q

From Proteopedia

Current revision

Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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