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5hnx

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==Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the nucleotide-free state==
==Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the nucleotide-free state==
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<StructureSection load='5hnx' size='340' side='right' caption='[[5hnx]], [[Resolution|resolution]] 6.60&Aring;' scene=''>
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<SX load='5hnx' size='340' side='right' viewer='molstar' caption='[[5hnx]], [[Resolution|resolution]] 6.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5hnx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HNX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5hnx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HNX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.6&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hnw|5hnw]], [[5hny|5hny]], [[5hnz|5hnz]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hnx OCA], [http://pdbe.org/5hnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hnx RCSB], [http://www.ebi.ac.uk/pdbsum/5hnx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hnx ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hnx OCA], [https://pdbe.org/5hnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hnx RCSB], [https://www.ebi.ac.uk/pdbsum/5hnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hnx ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/NCD_DROME NCD_DROME]] NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.<ref>PMID:2146510</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
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[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Kinesin-14 is a unique minus-end-directed microtubule-based motor. A swinging motion of a class-specific N-terminal neck helix has been proposed to produce minus-end directionality. However, it is unclear how swinging of the neck helix is driven by ATP hydrolysis utilizing the highly conserved catalytic core among all kinesins. Here, using a motility assay, we show that in addition to the neck helix, the conserved five residues at the C-terminal region in kinesin-14, namely the neck mimic, are necessary to give kinesin-1 an ability to reverse its directionality toward the minus end of microtubules. Our structural analyses further demonstrate that the C-terminal neck mimic, in cooperation with conformational changes in the catalytic core during ATP binding, forms a kinesin-14 bundle with the N-terminal neck helix to swing toward the minus end of microtubules. Thus, the neck mimic plays a crucial role in coupling the chemical ATPase reaction with the mechanical cycle to produce the minus-end-directed motility of kinesin-14.
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Structural Basis of Backwards Motion in Kinesin-1-Kinesin-14 Chimera: Implication for Kinesin-14 Motility.,Yamagishi M, Shigematsu H, Yokoyama T, Kikkawa M, Sugawa M, Aoki M, Shirouzu M, Yajima J, Nitta R Structure. 2016 Aug 2;24(8):1322-34. doi: 10.1016/j.str.2016.05.021. Epub 2016, Jul 21. PMID:27452403<ref>PMID:27452403</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5hnx" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Tubulin|Tubulin]]
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*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Kikkawa, M]]
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[[Category: Drosophila melanogaster]]
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[[Category: Nitta, R]]
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[[Category: Large Structures]]
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[[Category: Shigematsu, H]]
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[[Category: Rattus norvegicus]]
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[[Category: Shirouzu, M]]
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[[Category: Kikkawa M]]
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[[Category: Yokoyama, T]]
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[[Category: Nitta R]]
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[[Category: Atpase]]
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[[Category: Shigematsu H]]
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[[Category: Kinesin]]
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[[Category: Shirouzu M]]
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[[Category: Kinesin-14]]
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[[Category: Yokoyama T]]
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[[Category: Microtubule]]
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[[Category: Structural protein-motor protein complex]]
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Current revision

Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the nucleotide-free state

5hnx, resolution 6.60Å

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