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| | ==Green-fluorescent variant of the non-fluorescent chromoprotein Rtms5== | | ==Green-fluorescent variant of the non-fluorescent chromoprotein Rtms5== |
| - | <StructureSection load='3vic' size='340' side='right' caption='[[3vic]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='3vic' size='340' side='right'caption='[[3vic]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vic]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Coral Coral]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VIC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vic]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Montipora_efflorescens Montipora efflorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VIC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=QFG:{(4E)-2-[(1E)-4-AMINO-4-OXOBUTANIMIDOYL]-4-BENZYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>QFG</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=QFG:{(4E)-2-[(1E)-4-AMINO-4-OXOBUTANIMIDOYL]-4-BENZYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>QFG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mou|1mou]], [[1mov|1mov]], [[2arl|2arl]], [[2p4m|2p4m]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vic OCA], [https://pdbe.org/3vic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vic RCSB], [https://www.ebi.ac.uk/pdbsum/3vic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vic ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vic OCA], [http://pdbe.org/3vic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vic RCSB], [http://www.ebi.ac.uk/pdbsum/3vic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vic ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NFCP_MONEF NFCP_MONEF]] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs.<ref>PMID:12595737</ref> | + | [https://www.uniprot.org/uniprot/NFCP_MONEF NFCP_MONEF] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs.<ref>PMID:12595737</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Coral]] | + | [[Category: Large Structures]] |
| - | [[Category: Battad, J M]] | + | [[Category: Montipora efflorescens]] |
| - | [[Category: Byres, E]] | + | [[Category: Battad JM]] |
| - | [[Category: Devenish, R J]] | + | [[Category: Byres E]] |
| - | [[Category: Prescott, M]] | + | [[Category: Devenish RJ]] |
| - | [[Category: Rossjohn, J]] | + | [[Category: Prescott M]] |
| - | [[Category: Traore, D A.K]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Wilce, M]] | + | [[Category: Traore DAK]] |
| - | [[Category: Acylimine]]
| + | [[Category: Wilce M]] |
| - | [[Category: Anthozoa]]
| + | |
| - | [[Category: Beta barrel]]
| + | |
| - | [[Category: Beta can]]
| + | |
| - | [[Category: Chromoprotein]]
| + | |
| - | [[Category: Fluorescent]]
| + | |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Gfp]]
| + | |
| - | [[Category: Peptide-derived chromophore]]
| + | |
| - | [[Category: Pigment]]
| + | |
| - | [[Category: Pocilloporin]]
| + | |
| Structural highlights
Function
NFCP_MONEF Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs.[1]
Publication Abstract from PubMed
Rtms5 is an deep blue weakly fluorescent GFP-like protein ([Formula: see text], 592 nm; [Formula: see text], 630nm; Phi(F), 0.004) that contains a (66)Gln-Tyr-Gly chromophore tripeptide sequence. We investigated the optical properties and structure of two variants, Rtms5(Y67F) and Rtms5(Y67F/H146S) in which the tyrosine at position 67 was substituted by a phenylalanine. Compared to the parent proteins the optical spectra for these new variants were significantly blue-shifted. Rtms5(Y67F) spectra were characterised by two absorbing species ([Formula: see text], 440 nm and 513 nm) and green fluorescence emission ([Formula: see text], 440 nm; [Formula: see text], 508 nm; Phi(F), 0.11), whilst Rtms5(Y67F/H146S) spectra were characterised by a single absorbing species ([Formula: see text], 440 nm) and a relatively high fluorescence quantum yield (Phi(F,) 0.75; [Formula: see text], 440 nm; [Formula: see text], 508 nm). The fluorescence emissions of each variant were remarkably stable over a wide range of pH (3-11). These are the first GFP-like proteins with green emissions (500-520 nm) that do not have a tyrosine at position 67. The X-ray crystal structure of each protein was determined to 2.2 A resolution and showed that the benzylidine ring of the chromophore, similar to the 4-hydroxybenzylidine ring of the Rtms5 parent, is non-coplanar and in the trans conformation. The results of chemical quantum calculations together with the structural data suggested that the 513 nm absorbing species in Rtms5(Y67F) results from an unusual form of the chromophore protonated at the acylimine oxygen. These are the first X-ray crystal structures for fluorescent proteins with a functional chromophore containing a phenylalanine at position 67.
A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure.,Battad JM, Traore DA, Byres E, Rossjohn J, Devenish RJ, Olsen S, Wilce MC, Prescott M PLoS One. 2012;7(10):e47331. doi: 10.1371/journal.pone.0047331. Epub 2012 Oct 10. PMID:23071789[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Beddoe T, Ling M, Dove S, Hoegh-Guldberg O, Devenish RJ, Prescott M, Rossjohn J. The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):597-9. Epub 2003, Feb 21. PMID:12595737
- ↑ Battad JM, Traore DA, Byres E, Rossjohn J, Devenish RJ, Olsen S, Wilce MC, Prescott M. A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure. PLoS One. 2012;7(10):e47331. doi: 10.1371/journal.pone.0047331. Epub 2012 Oct 10. PMID:23071789 doi:http://dx.doi.org/10.1371/journal.pone.0047331
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