1prv

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[[Image:1prv.jpg|left|200px]]
 
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{{Structure
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==PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING==
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|PDB= 1prv |SIZE=350|CAPTION= <scene name='initialview01'>1prv</scene>
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<StructureSection load='1prv' size='340' side='right'caption='[[1prv]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1prv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRV FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1prv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prv OCA], [https://pdbe.org/1prv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1prv RCSB], [https://www.ebi.ac.uk/pdbsum/1prv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1prv ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1pru|1PRU]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prv OCA], [http://www.ebi.ac.uk/pdbsum/1prv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1prv RCSB]</span>
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[https://www.uniprot.org/uniprot/PURR_ECOLI PURR_ECOLI] Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. In addition, it participates in the regulation or coregulation of genes involved in de novo pyrimidine nucleotide biosynthesis, salvage and uptake (pyrC, pyrD, carAB and codBA), and of several genes encoding enzymes necessary for nucleotide and polyamine biosynthesis (prsA, glyA, gcvTHP, speA, glnB). Binds to a 16-bp palindromic sequence located within the promoter region of pur regulon genes. The consensus binding sequence is 5'-ACGCAAACGTTTTCNT-3'. PurR is allosterically activated to bind its cognate DNA by binding the purine corepressors, hypoxanthine or guanine, thereby effecting transcription repression.<ref>PMID:2404765</ref> <ref>PMID:2211500</ref> <ref>PMID:1400170</ref> <ref>PMID:14741201</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1prv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1prv ConSurf].
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<div style="clear:both"></div>
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'''PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING'''
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==See Also==
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*[[Purine repressor|Purine repressor]]
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== References ==
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==Overview==
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<references/>
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BACKGROUND: The purine repressor (PurR) regulates genes that encode enzymes for purine biosynthesis. PurR has a two domain structure with an N-terminal DNA-binding domain (DBD) and a C-terminal corepressor-binding domain (CBD). The three dimensional structure of a ternary complex of PurR bound to both corepressor and a specific DNA sequence has recently been determined by X-ray crystallography. RESULTS: We have determined the solution structure of the PurR DBD by NMR. It contains three helices, with the first and second helices forming a helix-turn-helix motif. The tertiary structure of the three helices is very similar to that of the corresponding region in the ternary complex. The structure of the hinge helical region, however, which makes specific base contacts in the minor groove of DNA, is disordered in the DNA-free form. CONCLUSION: The stable formation of PurR hinge helices requires PurR dimerization, which brings the hinge regions proximal to each other. The dimerization of the hinge helices is likely to be controlled by the CBD dimerization interface, but is induced by specific-DNA binding.
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__TOC__
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</StructureSection>
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==About this Structure==
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1PRV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRV OCA].
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==Reference==
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Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain., Nagadoi A, Morikawa S, Nakamura H, Enari M, Kobayashi K, Yamamoto H, Sampei G, Mizobuchi K, Schumacher MA, Brennan RG, et al., Structure. 1995 Nov 15;3(11):1217-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8591032 8591032]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brennan, R G.]]
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[[Category: Brennan RG]]
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[[Category: Enari, M.]]
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[[Category: Enari M]]
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[[Category: Kobayashi, K.]]
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[[Category: Kobayashi K]]
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[[Category: Mizobuchi, K.]]
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[[Category: Mizobuchi K]]
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[[Category: Morikawa, S.]]
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[[Category: Morikawa S]]
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[[Category: Nagadoi, A.]]
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[[Category: Nagadoi A]]
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[[Category: Nakamura, H.]]
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[[Category: Nakamura H]]
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[[Category: Nishimura, Y.]]
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[[Category: Nishimura Y]]
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[[Category: Sampei, G.]]
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[[Category: Sampei G]]
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[[Category: Schumacher, M A.]]
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[[Category: Schumacher MA]]
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[[Category: Yamamoto, H.]]
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[[Category: Yamamoto H]]
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[[Category: purine repressor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:03:45 2008''
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Current revision

PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING

PDB ID 1prv

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