This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5h0s

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:48, 2 August 2023) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5h0s is ON HOLD until Paper Publication
+
==EM Structure of VP1A and VP1B==
 +
<SX load='5h0s' size='340' side='right' viewer='molstar' caption='[[5h0s]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5h0s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H0S FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h0s OCA], [https://pdbe.org/5h0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h0s RCSB], [https://www.ebi.ac.uk/pdbsum/5h0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h0s ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CAPSD_CPVBM CAPSD_CPVBM] Capsid protein self-assembles to form an icosahedral capsid with a pseudo T=2 symmetry, about 50 nm in diameter, and consisting of 120 capsid proteins. The capsid encapsulates the genomic RNA.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Single-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than 3.5A) cryo-EM structures reported to date were obtained by using 300kV transmission electron microscopes (TEMs). We report here the structures of a cypovirus capsid of 750-A diameter at 3.3-A resolution and of RNA-dependent RNA polymerase (RdRp) complexes within the capsid at 3.9-A resolution using a 200-kV TEM. The newly resolved structure revealed conformational changes of two subdomains in the RdRp. These conformational changes, which were involved in RdRp's switch from non-transcribing to transcribing mode, suggest that the RdRp may facilitate the unwinding of genomic double-stranded RNA. The possibility of 3-A resolution structural determinations for biological assemblies of relatively small sizes using cryo-EM at 200kV was discussed.
-
Authors:
+
Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.,Li X, Zhou N, Chen W, Zhu B, Wang X, Xu B, Wang J, Liu H, Cheng L J Mol Biol. 2017 Jan 6;429(1):79-87. doi: 10.1016/j.jmb.2016.11.025. Epub 2016, Dec 1. PMID:27914893<ref>PMID:27914893</ref>
-
Description:
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
 +
<div class="pdbe-citations 5h0s" style="background-color:#fffaf0;"></div>
 +
 
 +
==See Also==
 +
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</SX>
 +
[[Category: Bombyx mori cypovirus 1]]
 +
[[Category: Large Structures]]
 +
[[Category: Chen W]]
 +
[[Category: Cheng L]]
 +
[[Category: Li X]]
 +
[[Category: Liu H]]
 +
[[Category: Wang J]]
 +
[[Category: Wang X]]
 +
[[Category: Xu B]]
 +
[[Category: Zhou N]]
 +
[[Category: Zhu B]]

Current revision

EM Structure of VP1A and VP1B

5h0s, resolution 3.30Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h0s"
Personal tools