5h0s

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:48, 2 August 2023) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5h0s is ON HOLD until Paper Publication
+
==EM Structure of VP1A and VP1B==
 +
<SX load='5h0s' size='340' side='right' viewer='molstar' caption='[[5h0s]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5h0s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H0S FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h0s OCA], [https://pdbe.org/5h0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h0s RCSB], [https://www.ebi.ac.uk/pdbsum/5h0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h0s ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CAPSD_CPVBM CAPSD_CPVBM] Capsid protein self-assembles to form an icosahedral capsid with a pseudo T=2 symmetry, about 50 nm in diameter, and consisting of 120 capsid proteins. The capsid encapsulates the genomic RNA.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Single-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than 3.5A) cryo-EM structures reported to date were obtained by using 300kV transmission electron microscopes (TEMs). We report here the structures of a cypovirus capsid of 750-A diameter at 3.3-A resolution and of RNA-dependent RNA polymerase (RdRp) complexes within the capsid at 3.9-A resolution using a 200-kV TEM. The newly resolved structure revealed conformational changes of two subdomains in the RdRp. These conformational changes, which were involved in RdRp's switch from non-transcribing to transcribing mode, suggest that the RdRp may facilitate the unwinding of genomic double-stranded RNA. The possibility of 3-A resolution structural determinations for biological assemblies of relatively small sizes using cryo-EM at 200kV was discussed.
-
Authors:
+
Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.,Li X, Zhou N, Chen W, Zhu B, Wang X, Xu B, Wang J, Liu H, Cheng L J Mol Biol. 2017 Jan 6;429(1):79-87. doi: 10.1016/j.jmb.2016.11.025. Epub 2016, Dec 1. PMID:27914893<ref>PMID:27914893</ref>
-
Description:
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
 +
<div class="pdbe-citations 5h0s" style="background-color:#fffaf0;"></div>
 +
 
 +
==See Also==
 +
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</SX>
 +
[[Category: Bombyx mori cypovirus 1]]
 +
[[Category: Large Structures]]
 +
[[Category: Chen W]]
 +
[[Category: Cheng L]]
 +
[[Category: Li X]]
 +
[[Category: Liu H]]
 +
[[Category: Wang J]]
 +
[[Category: Wang X]]
 +
[[Category: Xu B]]
 +
[[Category: Zhou N]]
 +
[[Category: Zhu B]]

Current revision

EM Structure of VP1A and VP1B

5h0s, resolution 3.30Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h0s"
Personal tools