This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5hxy
From Proteopedia
(Difference between revisions)
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of XerA recombinase== | |
| + | <StructureSection load='5hxy' size='340' side='right'caption='[[5hxy]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hxy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum_DSM_1728 Thermoplasma acidophilum DSM 1728]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HXY FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hxy OCA], [https://pdbe.org/5hxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hxy RCSB], [https://www.ebi.ac.uk/pdbsum/5hxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hxy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9HIM5_THEAC Q9HIM5_THEAC] Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.[HAMAP-Rule:MF_02055] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Site-specific Xer recombination plays a pivotal role in reshuffling genetic information. Here, we report the 2.5 A crystal structure of XerA from the archaean Thermoplasma acidophilum. Crystallographic data reveal a uniquely open conformational state, resulting in a C-shaped clamp with an angle of ~ 48 degrees and a distance of 57 A between the core-binding and the catalytic domains. The catalytic nucleophile, Tyr264, is positioned in cis-cleavage mode by XerA's C-term tail that interacts with the CAT domain of a neighboring monomer without DNA substrate. Structural comparisons of tyrosine recombinases elucidate the dynamics of Xer recombinase. | ||
| - | + | Crystal structure of Thermoplasma acidophilum XerA recombinase shows large C-shape clamp conformation and cis-cleavage mode for nucleophilic tyrosine.,Jo CH, Kim J, Han AR, Park SY, Hwang KY, Nam KH FEBS Lett. 2016 Mar;590(6):848-56. doi: 10.1002/1873-3468.12109. Epub 2016 Mar 4. PMID:26919387<ref>PMID:26919387</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Hwang | + | <div class="pdbe-citations 5hxy" style="background-color:#fffaf0;"></div> |
| - | [[Category: Nam | + | |
| + | ==See Also== | ||
| + | *[[Resolvase 3D structures|Resolvase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermoplasma acidophilum DSM 1728]] | ||
| + | [[Category: Hwang KY]] | ||
| + | [[Category: Nam KH]] | ||
Current revision
Crystal structure of XerA recombinase
| |||||||||||
