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| - | {{Large structure}}
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| | ==Structure of the bacterial sex F pilus (13.2 Angstrom rise)== | | ==Structure of the bacterial sex F pilus (13.2 Angstrom rise)== |
| - | <StructureSection load='5ler' size='340' side='right' caption='[[5ler]], [[Resolution|resolution]] 5.00Å' scene=''> | + | <SX load='5ler' size='340' side='right' viewer='molstar' caption='[[5ler]], [[Resolution|resolution]] 5.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ler]] is a 75 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LER FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ler]] is a 75 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LER FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6V6:[(2~{S})-3-[[(2~{R})-2,3-BIS(OXIDANYL)PROPOXY]-OXIDANYL-PHOSPHORYL]OXY-2-HEXADEC-9-ENOYLOXY-PROPYL]+HEXADECANOATE'>6V6</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ler FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ler OCA], [http://pdbe.org/5ler PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ler RCSB], [http://www.ebi.ac.uk/pdbsum/5ler PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ler ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6V6:[(2~{S})-3-[[(2~{R})-2,3-BIS(OXIDANYL)PROPOXY]-OXIDANYL-PHOSPHORYL]OXY-2-HEXADEC-9-ENOYLOXY-PROPYL]+HEXADECANOATE'>6V6</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ler FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ler OCA], [https://pdbe.org/5ler PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ler RCSB], [https://www.ebi.ac.uk/pdbsum/5ler PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ler ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | {{Large structure}} | |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PIL2_ECOLX PIL2_ECOLX]] Propilin is the precursor of the pilus subunit, pilin, that forms conjugative pili, the filamentous surface appendages required for cell-to-cell contact during the earlier stages of bacterial conjugation, and that retract after contact is established. Mature pilin is assembled with the help of TraQ and TraX (By similarity). | + | [https://www.uniprot.org/uniprot/PIL1_ECOLI PIL1_ECOLI] Propilin is the precursor of the pilus subunit, pilin, that forms conjugative pili, the filamentous surface appendages required for cell-to-cell contact during the earlier stages of bacterial conjugation, and that retract after contact is established. Mature pilin is assembled with the help of TraQ and TraX (PubMed:1464628, PubMed:6090426). Functions as a receptor for CdiA-CT from E.cloacae and E.coli, although it is not clear if this is physiologically relevant (PubMed:24889811).<ref>PMID:1464628</ref> <ref>PMID:24889811</ref> <ref>PMID:6090426</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| - | [[Category: Costa, T R.D]] | + | [[Category: Large Structures]] |
| - | [[Category: Egelman, E H]] | + | [[Category: Costa TRD]] |
| - | [[Category: Ilangovan, I]] | + | [[Category: Egelman EH]] |
| - | [[Category: Redzej, A]] | + | [[Category: Ilangovan I]] |
| - | [[Category: Santini, J M]] | + | [[Category: Redzej A]] |
| - | [[Category: Smith, T K]] | + | [[Category: Santini JM]] |
| - | [[Category: Ukleja, M]] | + | [[Category: Smith TK]] |
| - | [[Category: Waksman, G]] | + | [[Category: Ukleja M]] |
| - | [[Category: Cell adhesion]]
| + | [[Category: Waksman G]] |
| - | [[Category: F-pilus conjugation type iv secretion system phospholipid]]
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| - | [[Category: Protein fibril]]
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| Structural highlights
Function
PIL1_ECOLI Propilin is the precursor of the pilus subunit, pilin, that forms conjugative pili, the filamentous surface appendages required for cell-to-cell contact during the earlier stages of bacterial conjugation, and that retract after contact is established. Mature pilin is assembled with the help of TraQ and TraX (PubMed:1464628, PubMed:6090426). Functions as a receptor for CdiA-CT from E.cloacae and E.coli, although it is not clear if this is physiologically relevant (PubMed:24889811).[1] [2] [3]
Publication Abstract from PubMed
Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 A resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection.
Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.,Costa TR, Ilangovan A, Ukleja M, Redzej A, Santini JM, Smith TK, Egelman EH, Waksman G Cell. 2016 Sep 8;166(6):1436-1444.e10. doi: 10.1016/j.cell.2016.08.025. PMID:27610568[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Paiva WD, Grossman T, Silverman PM. Characterization of F-pilin as an inner membrane component of Escherichia coli K12. J Biol Chem. 1992 Dec 25;267(36):26191-7 PMID:1464628
- ↑ Beck CM, Diner EJ, Kim JJ, Low DA, Hayes CS. The F pilus mediates a novel pathway of CDI toxin import. Mol Microbiol. 2014 Jul;93(2):276-90. doi: 10.1111/mmi.12658. Epub 2014 Jun 15. PMID:24889811 doi:http://dx.doi.org/10.1111/mmi.12658
- ↑ Frost LS, Paranchych W, Willetts NS. DNA sequence of the F traALE region that includes the gene for F pilin. J Bacteriol. 1984 Oct;160(1):395-401. PMID:6090426 doi:10.1128/jb.160.1.395-401.1984
- ↑ Costa TR, Ilangovan A, Ukleja M, Redzej A, Santini JM, Smith TK, Egelman EH, Waksman G. Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex. Cell. 2016 Sep 8;166(6):1436-1444.e10. doi: 10.1016/j.cell.2016.08.025. PMID:27610568 doi:http://dx.doi.org/10.1016/j.cell.2016.08.025
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