Cholesterol oxidase

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== Structural highlights ==
== Structural highlights ==
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There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/3'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/6'>FAD binding site</scene> (PDB entry [[1coy]]).<ref>PMID:8218217</ref>
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There are predominantly hydrophobic/non-polar residues in <scene name='48/486379/Cv/7'>steroid substrate binding site</scene> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}) in COX. <scene name='48/486379/Cv/8'>FAD binding site</scene> (PDB entry [[1coy]]).<ref>PMID:8218217</ref> Water molecules are shown as red spheres. <scene name='48/486379/Cv/9'>Whole active site</scene>.
</StructureSection>
</StructureSection>
==3D structure of cholesterol oxidase==
==3D structure of cholesterol oxidase==
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**[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />
**[[3cox]], [[1i19]] – BsCOX – ''Brevibacterium sterolicum''<br />
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**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]] – StCOX – ''Streptomyces''<br />
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**[[1b4v]], [[1mxt]], [[1n1p]], [[1n4u]], [[1n4v]], [[1n4w]], [[2gew]], [[4u2l]], [[4u2s]], [[4u2t]], [[5kwf]], [[4xxg]], [[4xwr]], [[4rek]] – StCOX – ''Streptomyces''<br />
**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />
**[[1b8s]], [[1cbo]], [[1cc2]], [[1ijh]], [[3b6d]], [[3cnj]], [[3gyi]], [[3gyj]] – StCOX (mutant)<br />
**[[3js8]] – COX – ''Chromobacterium''<br />
**[[3js8]] – COX – ''Chromobacterium''<br />

Current revision

Cholesterol oxidase complex with steroid substrate (PDB entry 1coy)

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3D structure of cholesterol oxidase

Updated on 12-May-2019


References

  1. Sagermann M, Ohtaki A, Newton K, Doukyu N. Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp. DS-1. J Struct Biol. 2010 Apr;170(1):32-40. Epub 2010 Jan 25. PMID:20102741 doi:10.1016/j.jsb.2010.01.012
  2. Li J, Vrielink A, Brick P, Blow DM. Crystal structure of cholesterol oxidase complexed with a steroid substrate: implications for flavin adenine dinucleotide dependent alcohol oxidases. Biochemistry. 1993 Nov 2;32(43):11507-15. PMID:8218217

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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