1q0s

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[[Image:1q0s.jpg|left|200px]]
 
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{{Structure
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==Binary Structure of T4DAM with AdoHcy==
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|PDB= 1q0s |SIZE=350|CAPTION= <scene name='initialview01'>1q0s</scene>, resolution 2.3&Aring;
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<StructureSection load='1q0s' size='340' side='right'caption='[[1q0s]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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<table><tr><td colspan='2'>[[1q0s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q0S FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= DAM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q0s OCA], [https://pdbe.org/1q0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q0s RCSB], [https://www.ebi.ac.uk/pdbsum/1q0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q0s ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1q0t|1Q0T]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q0s OCA], [http://www.ebi.ac.uk/pdbsum/1q0s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q0s RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/DMA_BPT4 DMA_BPT4] This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q0/1q0s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q0s ConSurf].
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<div style="clear:both"></div>
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'''Binary Structure of T4DAM with AdoHcy'''
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==See Also==
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*[[DNA adenine methylase|DNA adenine methylase]]
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__TOC__
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==Overview==
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</StructureSection>
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DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA.
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[[Category: Escherichia virus T4]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Cheng X]]
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1Q0S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0S OCA].
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[[Category: Dong A]]
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[[Category: Hattman S]]
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==Reference==
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[[Category: Horton JR]]
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Structure of the bacteriophage T4 DNA adenine methyltransferase., Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X, Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12937411 12937411]
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[[Category: Kossykh V]]
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[[Category: Enterobacteria phage t4]]
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[[Category: Schlagman SL]]
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[[Category: Single protein]]
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[[Category: Yang Z]]
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[[Category: Site-specific DNA-methyltransferase (adenine-specific)]]
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[[Category: Zhang X]]
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[[Category: Cheng, X.]]
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[[Category: Zhang XJ]]
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[[Category: Dong, A.]]
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[[Category: Zhou L]]
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[[Category: Hattman, S.]]
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[[Category: Horton, J R.]]
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[[Category: Kossykh, V.]]
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[[Category: Schlagman, S L.]]
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[[Category: Yang, Z.]]
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[[Category: Zhang, X.]]
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[[Category: Zhang, X J.]]
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[[Category: Zhou, L.]]
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[[Category: methyltransferase]]
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[[Category: t4dam]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:07:16 2008''
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Current revision

Binary Structure of T4DAM with AdoHcy

PDB ID 1q0s

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