1q1b

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[[Image:1q1b.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of E. coli MalK in the nucleotide-free form==
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|PDB= 1q1b |SIZE=350|CAPTION= <scene name='initialview01'>1q1b</scene>, resolution 2.80&Aring;
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<StructureSection load='1q1b' size='340' side='right'caption='[[1q1b]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1q1b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q1B FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE= MALK OR B4035 OR Z5633 OR ECS5018 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q1b OCA], [https://pdbe.org/1q1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q1b RCSB], [https://www.ebi.ac.uk/pdbsum/1q1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q1b ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1q12|1Q12]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q1b OCA], [http://www.ebi.ac.uk/pdbsum/1q1b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q1b RCSB]</span>
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[https://www.uniprot.org/uniprot/MALK_ECOLI MALK_ECOLI] Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Responsible for energy coupling to the transport system.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Crystal structure of E. coli MalK in the nucleotide-free form'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/1q1b_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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The ATPase components of ATP binding cassette (ABC) transporters power the transporters by binding and hydrolyzing ATP. Major conformational changes of an ATPase are revealed by crystal structures of MalK, the ATPase subunit of the maltose transporter from Escherichia coli, in three different dimeric configurations. While other nucleotide binding domains or subunits display low affinity for each other in the absence of the transmembrane segments, the MalK dimer is stabilized through interactions of the additional C-terminal domains. In the two nucleotide-free structures, the N-terminal nucleotide binding domains are separated to differing degrees, and the dimer is maintained through contacts of the C-terminal regulatory domains. In the ATP-bound form, the nucleotide binding domains make contact and two ATPs lie buried along the dimer interface. The two nucleotide binding domains of the dimer open and close like a pair of tweezers, suggesting a regulatory mechanism for ATPase activity that may be tightly coupled to translocation.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q1b ConSurf].
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1Q1B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1B OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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A tweezers-like motion of the ATP-binding cassette dimer in an ABC transport cycle., Chen J, Lu G, Lin J, Davidson AL, Quiocho FA, Mol Cell. 2003 Sep;12(3):651-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14527411 14527411]
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[[Category: Escherichia coli K-12]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Chen J]]
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[[Category: Chen, J.]]
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[[Category: Davidson AL]]
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[[Category: Davidson, A L.]]
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[[Category: Lin J]]
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[[Category: Lin, J.]]
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[[Category: Lu G]]
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[[Category: Lu, G.]]
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[[Category: Quiocho FA]]
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[[Category: Quiocho, F A.]]
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[[Category: nucleotide-free form]]
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[[Category: semi-open dimer]]
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[[Category: sugar transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:07:30 2008''
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Current revision

Crystal structure of E. coli MalK in the nucleotide-free form

PDB ID 1q1b

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