5mc6

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'''Unreleased structure'''
 
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The entry 5mc6 is ON HOLD
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==Cryo-EM structure of a native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae==
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<SX load='5mc6' size='340' side='right' viewer='molstar' caption='[[5mc6]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5mc6]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MC6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CT:HYPUSINE'>5CT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mc6 OCA], [https://pdbe.org/5mc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mc6 RCSB], [https://www.ebi.ac.uk/pdbsum/5mc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mc6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL15A_YEAST RL15A_YEAST]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ski2-Ski3-Ski8 (Ski) is a helicase complex functioning with the RNA-degrading exosome to mediate the 3'-5' messenger RNA (mRNA) decay in turnover and quality-control pathways. We report that the Ski complex directly associates with 80S ribosomes presenting a short mRNA 3' overhang. We determined the structure of an endogenous ribosome-Ski complex using cryo-electron microscopy (EM) with a local resolution of the Ski complex ranging from 4 angstroms (A) in the core to about 10 A for intrinsically flexible regions. Ribosome binding displaces the autoinhibitory domain of the Ski2 helicase, positioning it in an open conformation near the ribosomal mRNA entry tunnel. We observe that the mRNA 3' overhang is threaded directly from the small ribosomal subunit to the helicase channel of Ski2, primed for ongoing exosome-mediated 3'-5' degradation.
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Authors: Schmidt, C., Kowalinski, E., Shanmuganathan, V., Defenouillere, Q., Braunger, K., Heuer, A., Pech, M., Namane, A., Berninghausen, O., Fromont-Racine, M., Jacquier, A., Conti, E., Becker, T., Beckmann, R.
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The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex.,Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillere Q, Braunger K, Heuer A, Pech M, Namane A, Berninghausen O, Fromont-Racine M, Jacquier A, Conti E, Becker T, Beckmann R Science. 2016 Dec 16;354(6318):1431-1433. PMID:27980209<ref>PMID:27980209</ref>
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Description: Cryo-EM structure of a native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Kowalinski, E]]
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<div class="pdbe-citations 5mc6" style="background-color:#fffaf0;"></div>
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[[Category: Braunger, K]]
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[[Category: Defenouillere, Q]]
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==See Also==
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[[Category: Jacquier, A]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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[[Category: Namane, A]]
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*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
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[[Category: Beckmann, R]]
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== References ==
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[[Category: Shanmuganathan, V]]
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<references/>
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[[Category: Fromont-Racine, M]]
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__TOC__
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[[Category: Berninghausen, O]]
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</SX>
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[[Category: Pech, M]]
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[[Category: Large Structures]]
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[[Category: Conti, E]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Heuer, A]]
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[[Category: Becker T]]
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[[Category: Becker, T]]
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[[Category: Beckmann R]]
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[[Category: Schmidt, C]]
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[[Category: Berninghausen O]]
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[[Category: Braunger K]]
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[[Category: Conti E]]
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[[Category: Defenouillere Q]]
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[[Category: Fromont-Racine M]]
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[[Category: Heuer A]]
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[[Category: Jacquier A]]
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[[Category: Kowalinski E]]
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[[Category: Namane A]]
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[[Category: Pech M]]
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[[Category: Schmidt C]]
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[[Category: Shanmuganathan V]]

Current revision

Cryo-EM structure of a native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae

5mc6, resolution 3.80Å

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