We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.
5mf2
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5mf2 is ON HOLD Authors: Description: Category: Unreleased Structures) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Bacteriophage T5 distal tail protein pb9 co-crystallized with Tb-Xo4== | |
| + | <StructureSection load='5mf2' size='340' side='right'caption='[[5mf2]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mf2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T5 Escherichia virus T5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MF2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7MT:Tb-Xo4'>7MT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mf2 OCA], [https://pdbe.org/5mf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mf2 RCSB], [https://www.ebi.ac.uk/pdbsum/5mf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mf2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DIT_BPT5 DIT_BPT5] Distal tail protein which forms a hexameric ring located at the tail tube end.<ref>PMID:24198424</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Macromolecular crystallography suffers from two major issues: getting well-diffracting crystals and solving the phase problem inherent to large macromolecules. Here, we describe the first example of a lanthanide complex family named "crystallophore" (Xo4), which contributes to tackling both bottlenecks. This terbium complex, Tb-Xo4, is an appealing agent for biocrystallography, combining the exceptional phasing power of the Tb(iii) heavy atom with powerful nucleating properties, providing ready-to-use crystals for structure determination. Furthermore, protein/Tb-Xo4 co-crystals can be easily detected and discriminated from other crystalline by-products using luminescence. We demonstrate the potential of this additive for the crystallisation and structure determination of eight proteins, two of whose structures were unknown. | ||
| - | + | Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effects, phasing properties, and luminescence.,Engilberge S, Riobe F, Di Pietro S, Lassalle L, Coquelle N, Arnaud CA, Pitrat D, Mulatier JC, Madern D, Breyton C, Maury O, Girard E Chem Sci. 2017 Sep 1;8(9):5909-5917. doi: 10.1039/c7sc00758b. Epub 2017 Jun 6. PMID:29619195<ref>PMID:29619195</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5mf2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia virus T5]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Arnaud C-A]] | ||
| + | [[Category: Breyton C]] | ||
| + | [[Category: Coquelle N]] | ||
| + | [[Category: Di Pietro S]] | ||
| + | [[Category: Engilberge S]] | ||
| + | [[Category: Girard E]] | ||
| + | [[Category: Lassalle L]] | ||
| + | [[Category: Madern D]] | ||
| + | [[Category: Maury O]] | ||
| + | [[Category: Riobe F]] | ||
Current revision
Bacteriophage T5 distal tail protein pb9 co-crystallized with Tb-Xo4
| |||||||||||
Categories: Escherichia virus T5 | Large Structures | Arnaud C-A | Breyton C | Coquelle N | Di Pietro S | Engilberge S | Girard E | Lassalle L | Madern D | Maury O | Riobe F
