5mih

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'''Unreleased structure'''
 
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The entry 5mih is ON HOLD until Paper Publication
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==Crystal structure of the lectin LecA from Pseudomonas aeruginosa in complex with a phenyl-epoxy-galactopyranoside==
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<StructureSection load='5mih' size='340' side='right'caption='[[5mih]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5mih]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MIH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7NU:phenyl+6,7-anhydro-D-glycero-beta-D-galacto-heptopyranoside'>7NU</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ETE:2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>ETE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mih OCA], [https://pdbe.org/5mih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mih RCSB], [https://www.ebi.ac.uk/pdbsum/5mih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mih ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Biofilm formation by pathogenic bacteria is a hallmark of chronic infections. In many cases, lectins play key roles in establishing biofilms. The pathogen Pseudomonas aeruginosa often exhibiting various drug resistances employs its lectins LecA and LecB as virulence factors and biofilm building blocks. Therefore, inhibition of the function of these proteins is thought to have potential in developing 'pathoblockers' preventing biofilm formation and virulence. Here, we describe for the first time a covalent lectin inhibitor specific to a carbohydrate binding site. In addition we report its application in the LecA-specific in vitro imaging of biofilms formed by P. aeruginosa.
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Authors: Wagner, S., Hauk, D., Hofmann, M., Joachim, I., Sommer, R., Muller, R., Imberty, A., Varrot, A., Titz, A.
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Covalent lectin inhibition and application in bacterial biofilm imaging.,Wagner S, Hauck D, Hoffmann M, Sommer R, Joachim I, Muller R, Imberty A, Varrot A, Titz A Angew Chem Int Ed Engl. 2017 Sep 28. doi: 10.1002/anie.201709368. PMID:28960731<ref>PMID:28960731</ref>
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Description: Crystal structure of the lectin LecA from Pseudomonas aeruginosa in complex with a phenyl-epoxy-galactopyranoside
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Muller, R]]
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<div class="pdbe-citations 5mih" style="background-color:#fffaf0;"></div>
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[[Category: Varrot, A]]
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[[Category: Hofmann, M]]
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==See Also==
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[[Category: Titz, A]]
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*[[Galactose-binding lectin|Galactose-binding lectin]]
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[[Category: Imberty, A]]
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== References ==
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[[Category: Sommer, R]]
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<references/>
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[[Category: Wagner, S]]
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__TOC__
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[[Category: Hauk, D]]
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</StructureSection>
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[[Category: Joachim, I]]
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Hauk D]]
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[[Category: Hofmann M]]
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[[Category: Imberty A]]
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[[Category: Joachim I]]
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[[Category: Muller R]]
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[[Category: Sommer R]]
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[[Category: Titz A]]
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[[Category: Varrot A]]
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[[Category: Wagner S]]

Current revision

Crystal structure of the lectin LecA from Pseudomonas aeruginosa in complex with a phenyl-epoxy-galactopyranoside

PDB ID 5mih

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