5tvd
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5tvd is ON HOLD until Paper Publication Authors: Asojo, O.A. Description: Crystal structure of Tm16 Category: Unreleased Structures [[Category:...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Tm16== | |
| + | <StructureSection load='5tvd' size='340' side='right'caption='[[5tvd]], [[Resolution|resolution]] 1.73Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5tvd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichuris_muris Trichuris muris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TVD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TVD FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.734Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tvd OCA], [https://pdbe.org/5tvd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tvd RCSB], [https://www.ebi.ac.uk/pdbsum/5tvd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tvd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Trichuriasis is a disease of poverty for which excretory and secretory (ES) products that induce the protective immunity are being investigated as candidate vaccines antigens. In this study, ES products of T. muris and immune sera were produced. The immune sera recognized more than 20 proteins on a 2D-gel of ES products of T. muris adult worms. Tm16 was one of the proteins identified by mass spectrometry. Tm16 shares 57% sequence identity with Ov16, an immunodominant diagnostic antigen from Onchocerca volvulus. Recombinant Tm16 with a carboxyl terminal hexahistidine was produced using Pichia pastoris. Polyclonal antibodies against rTm16 were generated by one-prime and two-boost immunization of three female Balb/c mice with 25 mug of recombinant Tm16 emulsified with ISA720 adjuvant. These polyclonal antibodies confirmed that Tm16 is localized to the ES products and the soluble fraction of the adult worm. Additionally, the high-resolution crystal structure of Tm16 was solved by molecular replacement. Tm16 belongs to the phosphatidylethanolamine-binding-like protein (PEBP1) family and this is the first structure of a PEBP1 from a parasite. | ||
| - | + | Identification, Characterization, and Structure of Tm16 from Trichuris muris.,Liu Z, Kelleher A, Tabb S, Wei J, Pollet J, Hotez PJ, Bottazzi ME, Zhan B, Asojo OA J Parasitol Res. 2017;2017:4342789. doi: 10.1155/2017/4342789. Epub 2017 Aug 14. PMID:28884022<ref>PMID:28884022</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Asojo | + | <div class="pdbe-citations 5tvd" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Trichuris muris]] | ||
| + | [[Category: Asojo OA]] | ||
Current revision
Crystal structure of Tm16
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