1qdl

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[[Image:1qdl.jpg|left|200px]]
 
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{{Structure
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==THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS==
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|PDB= 1qdl |SIZE=350|CAPTION= <scene name='initialview01'>1qdl</scene>, resolution 2.500&Aring;
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<StructureSection load='1qdl' size='340' side='right'caption='[[1qdl]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1qdl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QDL FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qdl OCA], [https://pdbe.org/1qdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qdl RCSB], [https://www.ebi.ac.uk/pdbsum/1qdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qdl ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qdl OCA], [http://www.ebi.ac.uk/pdbsum/1qdl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qdl RCSB]</span>
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[https://www.uniprot.org/uniprot/TRPE_SACS2 TRPE_SACS2] Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.<ref>PMID:10449718</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qd/1qdl_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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Anthranilate synthase catalyzes the synthesis of anthranilate from chorismate and glutamine and is feedback-inhibited by tryptophan. The enzyme of the hyperthermophile Sulfolobus solfataricus has been crystallized in the absence of physiological ligands, and its three-dimensional structure has been determined at 2.5-A resolution with x-ray crystallography. It is a heterotetramer of anthranilate synthase (TrpE) and glutamine amidotransferase (TrpG) subunits, in which two TrpG:TrpE protomers associate mainly via the TrpG subunits. The small TrpG subunit (195 residues) has the known "triad" glutamine amidotransferase fold. The large TrpE subunit (421 residues) has a novel fold. It displays a cleft between two domains, the tips of which contact the TrpG subunit across its active site. Clusters of catalytically essential residues are located inside the cleft, spatially separated from clustered residues involved in feedback inhibition. The structure suggests a model in which chorismate binding triggers a relative movement of the two domain tips of the TrpE subunit, activating the TrpG subunit and creating a channel for passage of ammonia toward the active site of the TrpE subunit. Tryptophan presumably blocks this rearrangement, thus stabilizing the inactive states of both subunits. The structure of the TrpE subunit is a likely prototype for the related enzymes 4-amino 4-deoxychorismate synthase and isochorismate synthase.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qdl ConSurf].
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1QDL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QDL OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications., Knochel T, Ivens A, Hester G, Gonzalez A, Bauerle R, Wilmanns M, Kirschner K, Jansonius JN, Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9479-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10449718 10449718]
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__TOC__
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[[Category: Anthranilate synthase]]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Bauerle, R.]]
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[[Category: Bauerle R]]
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[[Category: Gonzalez, A.]]
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[[Category: Gonzalez A]]
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[[Category: Hester, G.]]
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[[Category: Hester G]]
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[[Category: Ivens, A.]]
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[[Category: Ivens A]]
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[[Category: Jansonius, J N.]]
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[[Category: Jansonius JN]]
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[[Category: Kirschner, K.]]
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[[Category: Kirschner K]]
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[[Category: Knoechel, T.]]
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[[Category: Knoechel T]]
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[[Category: Wilmanns, M.]]
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[[Category: Wilmanns M]]
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[[Category: allosteric interaction]]
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[[Category: anthranilate synthase]]
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[[Category: glutamine amidotransferase]]
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[[Category: tryptophan biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:12:15 2008''
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Current revision

THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS

PDB ID 1qdl

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