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| | ==Interactions between the Mal3 EB1-like domain and Dis1== | | ==Interactions between the Mal3 EB1-like domain and Dis1== |
| - | <StructureSection load='5m9e' size='340' side='right' caption='[[5m9e]], [[Resolution|resolution]] 2.83Å' scene=''> | + | <StructureSection load='5m9e' size='340' side='right'caption='[[5m9e]], [[Resolution|resolution]] 2.83Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5m9e]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M9E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5m9e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M9E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m9e OCA], [http://pdbe.org/5m9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m9e RCSB], [http://www.ebi.ac.uk/pdbsum/5m9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m9e ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.83Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m9e OCA], [https://pdbe.org/5m9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m9e RCSB], [https://www.ebi.ac.uk/pdbsum/5m9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m9e ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MAL3_SCHPO MAL3_SCHPO]] May play a role in regulating the integrity of microtubules possibly by influencing their stability. Involved in an anchoring mechanism to maintain tea2 and tip1 at growing microtubule ends. Strongly stimulates the ATPase activity of tea2.<ref>PMID:15177031</ref> <ref>PMID:15665379</ref> [[http://www.uniprot.org/uniprot/DIS1_SCHPO DIS1_SCHPO]] Has a role in sister chromatid separation.<ref>PMID:7628693</ref> | + | [https://www.uniprot.org/uniprot/DIS1_SCHPO DIS1_SCHPO] Has a role in sister chromatid separation.<ref>PMID:7628693</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Singleton, M R]] | + | [[Category: Large Structures]] |
| - | [[Category: Zakian, S]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Cell cycle]]
| + | [[Category: Singleton MR]] |
| - | [[Category: Coiled-coil]]
| + | [[Category: Zakian S]] |
| - | [[Category: Eb1 domain]] | + | |
| - | [[Category: Microtubule-binding]] | + | |
| Structural highlights
Function
DIS1_SCHPO Has a role in sister chromatid separation.[1]
Publication Abstract from PubMed
Dynamic microtubule plus ends interact with various intracellular target regions such as the cell cortex and the kinetochore. Two conserved families of microtubule plus-end tracking proteins, XMAP215/TOG and EB1, play pivotal roles in regulating microtubule dynamics. Here we study the functional interplay between fission yeast Dis1/XMAP215 and Mal3/EB1. Using an in vitro microscopy assay, we find that purified Dis1 autonomously tracks growing microtubule ends and is a bona fide microtubule polymerase. Mal3 recruits additional Dis1 to microtubule ends, explaining the synergistic enhancement of microtubule dynamicity by these proteins. A non-canonical binding motif in Dis1 mediates the interaction with Mal3. X-ray crystallography shows that this novel motif interacts in an unconventional configuration with the conserved hydrophobic cavity formed within the Mal3 C-terminal region that typically interacts with the canonical SXIP motif. Selectively perturbing the Mal3-Dis1 interaction in living cells demonstrates that it is important for accurate chromosome segregation. Whereas in some metazoans the EB1-XMAP215/TOG interaction requires an additional binding partner, fission yeast relies on a direct interaction, indicating evolutionary plasticity of this critical interaction module.
An unconventional interaction between Dis1/TOG and Mal3/EB1 promotes the fidelity of chromosome segregation.,Matsuo Y, Maurer SP, Yukawa M, Zakian S, Singleton MR, Surrey T, Toda T J Cell Sci. 2016 Nov 21. pii: jcs.197533. PMID:27872152[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nabeshima K, Kurooka H, Takeuchi M, Kinoshita K, Nakaseko Y, Yanagida M. p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites. Genes Dev. 1995 Jul 1;9(13):1572-85. PMID:7628693
- ↑ Matsuo Y, Maurer SP, Yukawa M, Zakian S, Singleton MR, Surrey T, Toda T. An unconventional interaction between Dis1/TOG and Mal3/EB1 promotes the fidelity of chromosome segregation. J Cell Sci. 2016 Nov 21. pii: jcs.197533. PMID:27872152 doi:http://dx.doi.org/10.1242/jcs.197533
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