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5lsj

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CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C delta-HEAD2 COMPLEX

Structural highlights

5lsj is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.25Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MIS12_HUMAN Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis (PubMed:12515822, PubMed:15502821, PubMed:16585270). Essential for proper kinetochore microtubule attachments (PubMed:23891108).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Kinetochores, multisubunit protein assemblies, connect chromosomes to spindle microtubules to promote chromosome segregation. The 10-subunit KMN assembly (comprising KNL1, MIS12, and NDC80 complexes, designated KNL1C, MIS12C, and NDC80C) binds microtubules and regulates mitotic checkpoint function through NDC80C and KNL1C, respectively. MIS12C, on the other hand, connects the KMN to the chromosome-proximal domain of the kinetochore through a direct interaction with CENP-C. The structural basis for this crucial bridging function of MIS12C is unknown. Here, we report crystal structures of human MIS12C associated with a fragment of CENP-C and unveil the role of Aurora B kinase in the regulation of this interaction. The structure of MIS12:CENP-C complements previously determined high-resolution structures of functional regions of NDC80C and KNL1C and allows us to build a near-complete structural model of the KMN assembly. Our work illuminates the structural organization of essential chromosome segregation machinery that is conserved in most eukaryotes.

Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.,Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A Cell. 2016 Nov 3;167(4):1028-1040.e15. doi: 10.1016/j.cell.2016.10.005. Epub 2016, Oct 27. PMID:27881301^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Goshima G, Kiyomitsu T, Yoda K, Yanagida M. Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway. J Cell Biol. 2003 Jan 6;160(1):25-39. Epub 2003 Jan 6. PMID:12515822 doi:http://dx.doi.org/10.1083/jcb.200210005
↑ Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 2004 Nov;6(11):1135-41. Epub 2004 Oct 24. PMID:15502821 doi:http://dx.doi.org/10.1038/ncb1187
↑ Kline SL, Cheeseman IM, Hori T, Fukagawa T, Desai A. The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation. J Cell Biol. 2006 Apr 10;173(1):9-17. Epub 2006 Apr 3. PMID:16585270 doi:http://dx.doi.org/10.1083/jcb.200509158
↑ Shrestha RL, Draviam VM. Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK. Curr Biol. 2013 Aug 19;23(16):1514-26. doi: 10.1016/j.cub.2013.06.040. Epub 2013 , Jul 25. PMID:23891108 doi:http://dx.doi.org/10.1016/j.cub.2013.06.040
↑ Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A. Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. Cell. 2016 Nov 3;167(4):1028-1040.e15. doi: 10.1016/j.cell.2016.10.005. Epub 2016, Oct 27. PMID:27881301 doi:http://dx.doi.org/10.1016/j.cell.2016.10.005

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lsj"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C delta-HEAD2 COMPLEX==
-<StructureSection load='5lsj' size='340' side='right' caption='[[5lsj]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
+<StructureSection load='5lsj' size='340' side='right'caption='[[5lsj]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lsj]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LSJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lsj]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LSJ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsj OCA], [http://pdbe.org/5lsj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsj RCSB], [http://www.ebi.ac.uk/pdbsum/5lsj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsj ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsj OCA], [https://pdbe.org/5lsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lsj RCSB], [https://www.ebi.ac.uk/pdbsum/5lsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NSL1_HUMAN NSL1_HUMAN]] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.<ref>PMID:16585270</ref>  [[http://www.uniprot.org/uniprot/MIS12_HUMAN MIS12_HUMAN]] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis (PubMed:12515822, PubMed:15502821, PubMed:16585270). Essential for proper kinetochore microtubule attachments (PubMed:23891108).<ref>PMID:12515822</ref> <ref>PMID:15502821</ref> <ref>PMID:16585270</ref> <ref>PMID:23891108</ref>  [[http://www.uniprot.org/uniprot/DSN1_HUMAN DSN1_HUMAN]] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.<ref>PMID:15502821</ref> <ref>PMID:16585270</ref>  [[http://www.uniprot.org/uniprot/PMF1_HUMAN PMF1_HUMAN]] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.<ref>PMID:10419538</ref> <ref>PMID:11256947</ref> <ref>PMID:15502821</ref> <ref>PMID:16585270</ref>  [[http://www.uniprot.org/uniprot/CENPC_HUMAN CENPC_HUMAN]] Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.<ref>PMID:19482874</ref> <ref>PMID:21529714</ref>
+[https://www.uniprot.org/uniprot/MIS12_HUMAN MIS12_HUMAN] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis (PubMed:12515822, PubMed:15502821, PubMed:16585270). Essential for proper kinetochore microtubule attachments (PubMed:23891108).<ref>PMID:12515822</ref> <ref>PMID:15502821</ref> <ref>PMID:16585270</ref> <ref>PMID:23891108</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 </div>
 <div class="pdbe-citations 5lsj" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Centromere protein 3D structure|Centromere protein 3D structure]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Keller, J]]
+[[Category: Homo sapiens]]
-[[Category: Liu, Y]]
+[[Category: Large Structures]]
-[[Category: Petrovic, A]]
+[[Category: Keller J]]
-[[Category: Vetter, I R]]
+[[Category: Liu Y]]
-[[Category: Alpha-helical]]
+[[Category: Petrovic A]]
-[[Category: Cell cycle]]
+[[Category: Vetter IR]]

5lsj

From Proteopedia

Current revision

CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C delta-HEAD2 COMPLEX

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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