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| | ==Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, K+ complex== | | ==Crystal Structure of CNG mimicking NaK mutant, NaK-DTPP, K+ complex== |
| - | <StructureSection load='3k03' size='340' side='right' caption='[[3k03]], [[Resolution|resolution]] 1.62Å' scene=''> | + | <StructureSection load='3k03' size='340' side='right'caption='[[3k03]], [[Resolution|resolution]] 1.62Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3k03]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K03 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3k03]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K03 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.62Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k04|3k04]], [[3k06|3k06]], [[3k08|3k08]], [[3k0d|3k0d]], [[3k0g|3k0g]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BC_0669 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 ATCC 14579])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k03 OCA], [https://pdbe.org/3k03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k03 RCSB], [https://www.ebi.ac.uk/pdbsum/3k03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k03 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k03 OCA], [http://pdbe.org/3k03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k03 RCSB], [http://www.ebi.ac.uk/pdbsum/3k03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k03 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q81HW2_BACCR Q81HW2_BACCR] |
| - | Selective ion conduction across ion channel pores is central to cellular physiology. To understand the underlying principles of ion selectivity in tetrameric cation channels, we engineered a set of cation channel pores based on the nonselective NaK channel and determined their structures to high resolution. These structures showcase an ensemble of selectivity filters with a various number of contiguous ion binding sites ranging from 2 to 4, with each individual site maintaining a geometry and ligand environment virtually identical to that of equivalent sites in K(+) channel selectivity filters. Combined with single channel electrophysiology, we show that only the channel with four ion binding sites is K(+) selective, whereas those with two or three are nonselective and permeate Na(+) and K(+) equally well. These observations strongly suggest that the number of contiguous ion binding sites in a single file is the key determinant of the channel's selectivity properties and the presence of four sites in K(+) channels is essential for highly selective and efficient permeation of K(+) ions.
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| - | Tuning the ion selectivity of tetrameric cation channels by changing the number of ion binding sites.,Derebe MG, Sauer DB, Zeng W, Alam A, Shi N, Jiang Y Proc Natl Acad Sci U S A. 2010 Dec 27. PMID:21187421<ref>PMID:21187421</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3k03" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Potassium Channel|Potassium Channel]] | + | *[[Potassium channel 3D structures|Potassium channel 3D structures]] |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 14579]] | + | [[Category: Bacillus cereus]] |
| - | [[Category: Derebe, M G]] | + | [[Category: Large Structures]] |
| - | [[Category: Jiang, Y]] | + | [[Category: Derebe MG]] |
| - | [[Category: Cng channel selectivity filter]] | + | [[Category: Jiang Y]] |
| - | [[Category: Cng mimicking]]
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| - | [[Category: Dtpp]]
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| - | [[Category: Ionic channel]]
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| - | [[Category: Nak]]
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| - | [[Category: Nak-dtpp]]
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| - | [[Category: Nak-mutant]]
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| - | [[Category: Transport protein]]
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