This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5tcs

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:40, 6 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Crystal structure of a Dwarf Ndc80 Tetramer==
==Crystal structure of a Dwarf Ndc80 Tetramer==
-
<StructureSection load='5tcs' size='340' side='right' caption='[[5tcs]], [[Resolution|resolution]] 2.83&Aring;' scene=''>
+
<StructureSection load='5tcs' size='340' side='right'caption='[[5tcs]], [[Resolution|resolution]] 2.83&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5tcs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TCS FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5tcs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TCS FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8313&#8491;</td></tr>
-
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcs OCA], [http://pdbe.org/5tcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tcs RCSB], [http://www.ebi.ac.uk/pdbsum/5tcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcs ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcs OCA], [https://pdbe.org/5tcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tcs RCSB], [https://www.ebi.ac.uk/pdbsum/5tcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcs ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref> [[http://www.uniprot.org/uniprot/NUF2_YEAST NUF2_YEAST]] Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> [[http://www.uniprot.org/uniprot/NDC80_YEAST NDC80_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:10409732</ref> <ref>PMID:11266451</ref> <ref>PMID:12514103</ref> <ref>PMID:9585415</ref> [[http://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref>
+
[https://www.uniprot.org/uniprot/NDC80_YEAST NDC80_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:10409732</ref> <ref>PMID:11266451</ref> <ref>PMID:12514103</ref> <ref>PMID:9585415</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
The heterotetrameric Ndc80 complex establishes connectivity along the principal longitudinal axis of a kinetochore. Its two heterodimeric subcomplexes, each with a globular end and a coiled-coil shaft, connect end-to-end to create a approximately 600 A long rod spanning the gap from centromere-proximal structures to spindle microtubules. Neither subcomplex has a known function on its own, but the heterotetrameric organization and the characteristics of the junction are conserved from yeast to man. We have determined crystal structures of two shortened ("dwarf") Ndc80 complexes that contain the full tetramer junction and both globular ends. The junction connects two alpha-helical coiled coils through regions of four-chain and three-chain overlap. The complexity of its structure depends on interactions among conserved amino-acid residues, suggesting a binding site for additional cellular factor(s) not yet identified.
+
-
 
+
-
Conserved Tetramer Junction in the Kinetochore Ndc80 Complex.,Valverde R, Ingram J, Harrison SC Cell Rep. 2016 Nov 15;17(8):1915-1922. doi: 10.1016/j.celrep.2016.10.065. PMID:27851957<ref>PMID:27851957</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5tcs" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Harrison, S C]]
+
[[Category: Large Structures]]
-
[[Category: Valverde, R]]
+
[[Category: Saccharomyces cerevisiae S288C]]
-
[[Category: Ch]]
+
[[Category: Harrison SC]]
-
[[Category: Coiled-coil]]
+
[[Category: Valverde R]]
-
[[Category: Kinetochore]]
+
-
[[Category: Ndc80]]
+
-
[[Category: Replication]]
+
-
[[Category: Rwd]]
+
-
[[Category: Tetramer]]
+

Current revision

Crystal structure of a Dwarf Ndc80 Tetramer

PDB ID 5tcs

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5tcs"
Personal tools