|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Imipenem acylated BlaR1 sensor domain from Staphylococcus aureus== | | ==Imipenem acylated BlaR1 sensor domain from Staphylococcus aureus== |
| - | <StructureSection load='3q81' size='340' side='right' caption='[[3q81]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3q81' size='340' side='right'caption='[[3q81]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3q81]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q81 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3q81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q81 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IM2:(5R)-5-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-[(2-{[(E)-IMINOMETHYL]AMINO}ETHYL)SULFANYL]-4,5-DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>IM2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xa1|1xa1]], [[3q7v|3q7v]], [[3q7z|3q7z]], [[3q82|3q82]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IM2:(5R)-5-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-[(2-{[(E)-IMINOMETHYL]AMINO}ETHYL)SULFANYL]-4,5-DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>IM2</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaR1, VRA0048 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q81 OCA], [https://pdbe.org/3q81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q81 RCSB], [https://www.ebi.ac.uk/pdbsum/3q81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q81 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q81 OCA], [http://pdbe.org/3q81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q81 RCSB], [http://www.ebi.ac.uk/pdbsum/3q81 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q81 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/BLAR_STAAU BLAR_STAAU] BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker, B M]] | + | [[Category: Large Structures]] |
| - | [[Category: Borbulevych, O Y]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Mobashery, S]] | + | [[Category: Baker BM]] |
| - | [[Category: Antibiotic-binding]] | + | [[Category: Borbulevych OY]] |
| - | [[Category: Antibiotic-binding-antibiotic complex]] | + | [[Category: Mobashery S]] |
| - | [[Category: Hydrolase regulator-antibiotic complex]]
| + | |
| - | [[Category: Mrsa]]
| + | |
| Structural highlights
Function
BLAR_STAAU BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase.
Publication Abstract from PubMed
The integral membrane protein BlaR1 of methicillin-resistant Staphylococcus aureus senses the presence of beta-lactam antibiotics in the milieu and transduces the information to the cytoplasm, where the biochemical events that unleash induction of antibiotic resistance mechanisms take place. We report herein by two-dimensional and three-dimensional NMR experiments of the sensor domain of BlaR1 in solution and by determination of an x-ray structure for the apo protein that Lys-392 of the antibiotic-binding site is posttranslationally modified by N(zeta)-carboxylation. Additional crystallographic and NMR data reveal that on acylation of Ser-389 by antibiotics, Lys-392 experiences N(zeta)-decarboxylation. This unique process, termed the lysine N(zeta)-decarboxylation switch, arrests the sensor domain in the activated ("on") state, necessary for signal transduction and all the subsequent biochemical processes. We present structural information on how this receptor activation process takes place, imparting longevity to the antibiotic-receptor complex that is needed for the induction of the antibiotic-resistant phenotype in methicillin-resistant S. aureus.
Lysine Nzeta-decarboxylation switch and activation of the beta-lactam sensor domain of BlaR1 protein of methicillin-resistant Staphylococcus aureus.,Borbulevych O, Kumarasiri M, Wilson B, Llarrull LI, Lee M, Hesek D, Shi Q, Peng J, Baker BM, Mobashery S J Biol Chem. 2011 Sep 9;286(36):31466-72. Epub 2011 Jul 20. PMID:21775440[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Borbulevych O, Kumarasiri M, Wilson B, Llarrull LI, Lee M, Hesek D, Shi Q, Peng J, Baker BM, Mobashery S. Lysine Nzeta-decarboxylation switch and activation of the beta-lactam sensor domain of BlaR1 protein of methicillin-resistant Staphylococcus aureus. J Biol Chem. 2011 Sep 9;286(36):31466-72. Epub 2011 Jul 20. PMID:21775440 doi:http://dx.doi.org/10.1074/jbc.M111.252189
|
