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| | ==RedJ-Thioesterase from the Prodiginine biosynthetic pathway in Streptomyces coelicolor== | | ==RedJ-Thioesterase from the Prodiginine biosynthetic pathway in Streptomyces coelicolor== |
| - | <StructureSection load='3qmv' size='340' side='right' caption='[[3qmv]], [[Resolution|resolution]] 2.12Å' scene=''> | + | <StructureSection load='3qmv' size='340' side='right'caption='[[3qmv]], [[Resolution|resolution]] 2.12Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qmv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QMV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QMV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qmv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QMV FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qmw|3qmw]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCO5894, SC3F7.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qmv OCA], [https://pdbe.org/3qmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qmv RCSB], [https://www.ebi.ac.uk/pdbsum/3qmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qmv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qmv OCA], [http://pdbe.org/3qmv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qmv RCSB], [http://www.ebi.ac.uk/pdbsum/3qmv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qmv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| - | *[[Thioesterase|Thioesterase]] | + | *[[Thioesterase 3D structures|Thioesterase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Smith, J L]] | + | [[Category: Large Structures]] |
| - | [[Category: Whicher, J R]] | + | [[Category: Streptomyces coelicolor]] |
| - | [[Category: Alpha/beta hydrolase fold]] | + | [[Category: Smith JL]] |
| - | [[Category: Hydrolase]] | + | [[Category: Whicher JR]] |
| Structural highlights
Publication Abstract from PubMed
Prodiginines are a class of red-pigmented natural products with immunosuppressant, anticancer and antimalarial activities. Recent studies on prodiginine biosynthesis in Streptomyces coelicolor have elucidated the function of many enzymes within the pathway. However, the function of RedJ, which was predicted to be an editing thioesterase based on sequence similarity, is unknown. We report here the genetic, biochemical, and structural characterization of the redJ gene product. Deletion of redJ in S. coelicolor leads to a 75% decrease in prodiginine production, demonstrating its importance for prodiginine biosynthesis. RedJ exhibits thioesterase activity with selectivity for substrates having long acyl chains and lacking a beta-carboxyl substituent. The thioesterase has 1000-fold greater catalytic efficiency with substrates linked to an acyl carrier protein (ACP) than with the corresponding CoA thioester substrates. Also, RedJ strongly discriminates against the streptomycete ACP of fatty acid biosynthesis in preference to RedQ, an ACP of the prodiginine pathway. The 2.12-A crystal structure of RedJ provides insights into the molecular basis for the observed substrate selectivity. A hydrophobic pocket in the active site chamber is positioned to bind long acyl chains, as suggested by a long-chain ligand from the crystallization solution bound in this pocket. The accessibility of the active site is controlled by the position of a highly flexible entrance flap. These data combined with previous studies of prodiginine biosynthesis in S. coelicolor support a novel role for RedJ in facilitating transfer of a dodecanoyl chain from one acyl carrier protein to another en route to the key biosynthetic intermediate 2-undecylpyrrole.
Structure and Function of RedJ, a thioesterase from the prodiginine biosynthetic pathway in Streptomyces coelicolor.,Whicher JR, Florova G, Sydor PK, Singh R, Alhamadsheh M, Challis GL, Reynolds KA, Smith JL J Biol Chem. 2011 May 3. PMID:21543318[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Whicher JR, Florova G, Sydor PK, Singh R, Alhamadsheh M, Challis GL, Reynolds KA, Smith JL. Structure and Function of RedJ, a thioesterase from the prodiginine biosynthetic pathway in Streptomyces coelicolor. J Biol Chem. 2011 May 3. PMID:21543318 doi:10.1074/jbc.M110.213512
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