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| | ==Crystal Structure of rBTI-trypsin complex at 2.26 angstrom resolution== | | ==Crystal Structure of rBTI-trypsin complex at 2.26 angstrom resolution== |
| - | <StructureSection load='3rdz' size='340' side='right' caption='[[3rdz]], [[Resolution|resolution]] 2.26Å' scene=''> | + | <StructureSection load='3rdz' size='340' side='right'caption='[[3rdz]], [[Resolution|resolution]] 2.26Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rdz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Common_buckwheat Common buckwheat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RDZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rdz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Fagopyrum_esculentum Fagopyrum esculentum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RDZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.262Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rdy|3rdy]], [[2cmy|2cmy]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BWI-1, Edn1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3617 Common buckwheat])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdz OCA], [https://pdbe.org/3rdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rdz RCSB], [https://www.ebi.ac.uk/pdbsum/3rdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdz ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdz OCA], [http://pdbe.org/3rdz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rdz RCSB], [http://www.ebi.ac.uk/pdbsum/3rdz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Trypsin|Trypsin]]
| + | *[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] |
| - | *[[Trypsin inhibitor|Trypsin inhibitor]] | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bos taurus]] | | [[Category: Bos taurus]] |
| - | [[Category: Common buckwheat]] | + | [[Category: Fagopyrum esculentum]] |
| - | [[Category: Trypsin]] | + | [[Category: Large Structures]] |
| - | [[Category: Chang, W R]] | + | [[Category: Chang WR]] |
| - | [[Category: Li, M]] | + | [[Category: Li M]] |
| - | [[Category: Wang, L F]] | + | [[Category: Wang LF]] |
| - | [[Category: Hydrolase-hydrolase inhibitor complex]]
| + | |
| - | [[Category: Potato inhibitor i]]
| + | |
| - | [[Category: Serine protease inhibitor]]
| + | |
| - | [[Category: Trypsin inhibitor]]
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| Structural highlights
Function
TRY1_BOVIN
Publication Abstract from PubMed
BWI-1 (buckwheat trypsin inhibitor), a member of the potato inhibitor I family, suppresses the growth of T-acute lymphoblastic leukemia cells and induces apoptosis in human solid tumor cell lines. Here, we report the crystal structure of rBTI (recombinant buckwheat trypsin inhibitor), a recombinant protein of BWI-1, at 1.84 A resolution and the structure of rBTI in complex with bovine trypsin at 2.26 A resolution. A conformational change of Trp53 at the P(8)' position in rBTI was observed upon its binding to trypsin, which is not seen in other members of the potato inhibitor I family reported previously. The role of the P(8)' residue in the potato inhibitor I family was examined by measuring the association and dissociation rates of four rBTI mutants with different substitutions at the P(2) and P(8)' positions when binding to trypsin. One of the mutants, P44T, was found to be a much stronger inhibitor than wild-type rBTI, with a picomolar (pM) dissociation constant. Our results could provide valuable insights for designing a new rBTI-based antitumor drug in the future.
Conformational Changes of rBTI from Buckwheat upon Binding to Trypsin: Implications for the Role of the P(8)' Residue in the Potato Inhibitor I Family.,Wang L, Zhao F, Li M, Zhang H, Gao Y, Cao P, Pan X, Wang Z, Chang W PLoS One. 2011;6(6):e20950. Epub 2011 Jun 15. PMID:21698291[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wang L, Zhao F, Li M, Zhang H, Gao Y, Cao P, Pan X, Wang Z, Chang W. Conformational Changes of rBTI from Buckwheat upon Binding to Trypsin: Implications for the Role of the P(8)' Residue in the Potato Inhibitor I Family. PLoS One. 2011;6(6):e20950. Epub 2011 Jun 15. PMID:21698291 doi:10.1371/journal.pone.0020950
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