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| | ==Crystal structure of mAb 17.2 in complex with R13 peptide== | | ==Crystal structure of mAb 17.2 in complex with R13 peptide== |
| - | <StructureSection load='3sge' size='340' side='right' caption='[[3sge]], [[Resolution|resolution]] 1.89Å' scene=''> | + | <StructureSection load='3sge' size='340' side='right'caption='[[3sge]], [[Resolution|resolution]] 1.89Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SGE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SGE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sgd|3sgd]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [http://pdbe.org/3sge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [http://www.ebi.ac.uk/pdbsum/3sge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sge ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [https://pdbe.org/3sge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [https://www.ebi.ac.uk/pdbsum/3sge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sge ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </div> | | </div> |
| | <div class="pdbe-citations 3sge" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 3sge" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Bentley, G A]] | + | [[Category: Mus musculus]] |
| - | [[Category: Boulot, G]] | + | [[Category: Bentley GA]] |
| - | [[Category: Hontebeyrie, M]] | + | [[Category: Boulot G]] |
| - | [[Category: Pizarro, J C]] | + | [[Category: Hontebeyrie M]] |
| - | [[Category: Antigen binding]] | + | [[Category: Pizarro JC]] |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Patients with Chronic Chagas' Heart Disease possess high levels of antibodies against the carboxyl-terminal end of the ribosomal P2ss protein of Trypanosoma cruzi (TcP2ss). These antibodies, as well as the murine monoclonal antibody (mAb) 17.2, recognize the last 13 amino acids of TcP2ss (called the R13 epitope: EEEDDDMGFGLFD) and are able to cross-react with, and stimulate, the ss1 adrenergic receptor (ss1-AR). Indeed, the mAb 17.2 was able to specifically detect human beta1-AR, stably transfected into HEK cells, by flow cytometry and to induce repolarisation abnormalities and first degree atrioventricular conduction block after passive transfer to naive mice. To study the structural basis of this cross-reactivity, we determined the crystal structure of the Fab region of the mAb 17.2 alone at 2.31 A resolution and in complex with the R13 peptide at 1.89 A resolution. We identified as key contact residues on R13 peptide Glu3, Asp6 and Phe9 as was previously shown by alanine scanning. Additionally, we generated a model of human beta1-AR to elucidate the interaction with anti-R13 antibodies. These data provide an understanding of the molecular basis of cross-reactive antibodies induced by chronic infection with Trypanosoma cruzi.
Crystal structure of the complex mAb 17.2 and the C-terminal region of Trypanosoma cruzi P2beta protein: implications in cross-reactivity.,Pizarro JC, Boulot G, Bentley GA, Gomez KA, Hoebeke J, Hontebeyrie M, Levin MJ, Smulski CR PLoS Negl Trop Dis. 2011 Nov;5(11):e1375. doi: 10.1371/journal.pntd.0001375. Epub, 2011 Nov 1. PMID:22069505[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pizarro JC, Boulot G, Bentley GA, Gomez KA, Hoebeke J, Hontebeyrie M, Levin MJ, Smulski CR. Crystal structure of the complex mAb 17.2 and the C-terminal region of Trypanosoma cruzi P2beta protein: implications in cross-reactivity. PLoS Negl Trop Dis. 2011 Nov;5(11):e1375. doi: 10.1371/journal.pntd.0001375. Epub, 2011 Nov 1. PMID:22069505 doi:http://dx.doi.org/10.1371/journal.pntd.0001375
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