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| | ==Crystal Structure Analysis of Polyporus squamosus lectin bound to human-type influenza-binding epitope Neu5Aca2-6Galb1-4GlcNAc== | | ==Crystal Structure Analysis of Polyporus squamosus lectin bound to human-type influenza-binding epitope Neu5Aca2-6Galb1-4GlcNAc== |
| - | <StructureSection load='3phz' size='340' side='right' caption='[[3phz]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='3phz' size='340' side='right'caption='[[3phz]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3phz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Polyporus_squamosus Polyporus squamosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PHZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3phz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerioporus_squamosus Cerioporus squamosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PHZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">psl1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5640 Polyporus squamosus])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900046:6-sialyl-N-acetyllactosamine'>PRD_900046</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3phz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phz OCA], [http://pdbe.org/3phz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3phz RCSB], [http://www.ebi.ac.uk/pdbsum/3phz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3phz ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3phz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3phz OCA], [https://pdbe.org/3phz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3phz RCSB], [https://www.ebi.ac.uk/pdbsum/3phz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3phz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q75WT9_9APHY Q75WT9_9APHY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Ricin|Ricin]] | + | *[[Ricin 3D structures|Ricin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Polyporus squamosus]] | + | [[Category: Cerioporus squamosus]] |
| - | [[Category: Kadirvelraj, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Woods, R J]] | + | [[Category: Kadirvelraj R]] |
| - | [[Category: 6-sialyl-lactosamine]] | + | [[Category: Woods RJ]] |
| - | [[Category: Beta trefoil]]
| + | |
| - | [[Category: Saccharide binding lectin]]
| + | |
| - | [[Category: Sugar binding protein]]
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| Structural highlights
Function
Q75WT9_9APHY
Publication Abstract from PubMed
Glycan chains that terminate in sialic acid (Neu5Ac) are frequently the receptors targeted by pathogens for initial adhesion. Carbohydrate-binding proteins (lectins) with specificity for Neu5Ac are particularly useful in the detection and isolation of sialylated glycoconjugates, such as those associated with pathogen adhesion as well as those characteristic of several diseases including cancer. Structural studies of lectins are essential in order to understand the origin of their specificity, which is particularly important when employing such reagents as diagnostic tools. Here, we report a crystallographic and molecular dynamics (MD) analysis of a lectin from Polyporus squamosus (PSL) that is specific for glycans terminating with the sequence Neu5Acalpha2-6Galbeta. Because of its importance as a histological reagent, the PSL structure was solved (to 1.7 A) in complex with a trisaccharide, whose sequence (Neu5Acalpha2-6Galbeta1-4GlcNAc) is exploited by influenza A hemagglutinin for viral adhesion to human tissue. The structural data illuminate the origin of the high specificity of PSL for the Neu5Acalpha2-6Gal sequence. Theoretical binding free energies derived from the MD data confirm the key interactions identified crystallographically and provide additional insight into the relative contributions from each amino acid, as well as estimates of the importance of entropic and enthalpic contributions to binding.
Structure and binding analysis of Polyporus squamosus lectin in complex with the Neu5Ac{alpha}2-6Gal{beta}1-4GlcNAc human-type influenza receptor.,Kadirvelraj R, Grant OC, Goldstein IJ, Winter HC, Tateno H, Fadda E, Woods RJ Glycobiology. 2011 Jul;21(7):973-84. Epub 2011 Mar 24. PMID:21436237[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kadirvelraj R, Grant OC, Goldstein IJ, Winter HC, Tateno H, Fadda E, Woods RJ. Structure and binding analysis of Polyporus squamosus lectin in complex with the Neu5Ac{alpha}2-6Gal{beta}1-4GlcNAc human-type influenza receptor. Glycobiology. 2011 Jul;21(7):973-84. Epub 2011 Mar 24. PMID:21436237 doi:10.1093/glycob/cwr030
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