4y9w
From Proteopedia
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==Aspartic Proteinase Sapp2 Secreted from Candida Parapsilosis at 0.82 A Resolution.== | ==Aspartic Proteinase Sapp2 Secreted from Candida Parapsilosis at 0.82 A Resolution.== | ||
| - | <StructureSection load='4y9w' size='340' side='right' caption='[[4y9w]], [[Resolution|resolution]] 0.83Å' scene=''> | + | <StructureSection load='4y9w' size='340' side='right'caption='[[4y9w]], [[Resolution|resolution]] 0.83Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4y9w]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[4y9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y9W FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IVA:ISOVALERIC+ACID'>IVA</scene>, <scene name='pdbligand=STA:STATINE'>STA</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y9w OCA], [https://pdbe.org/4y9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y9w RCSB], [https://www.ebi.ac.uk/pdbsum/4y9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y9w ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G8B6Y8_CANPC G8B6Y8_CANPC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The virulence of the Candida pathogens is enhanced by the production of secreted aspartic proteases, which therefore represent possible targets for drug design. Here, the crystal structure of the secreted aspartic protease Sapp2p from Candida parapsilosis was determined. Sapp2p was isolated from its natural source and crystallized in complex with pepstatin A, a classical aspartic protease inhibitor. The atomic resolution of 0.83 A allowed the protonation states of the active-site residues to be inferred. A detailed comparison of the structure of Sapp2p with the structure of Sapp1p, the most abundant C. parapsilosis secreted aspartic protease, was performed. The analysis, which included advanced quantum-chemical interaction-energy calculations, uncovered molecular details that allowed the experimentally observed equipotent inhibition of both isoenzymes by pepstatin A to be rationalized. | ||
| + | |||
| + | Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.,Dostal J, Pecina A, Hruskova-Heidingsfeldova O, Mareckova L, Pichova I, Rezacova P, Lepsik M, Brynda J Acta Crystallogr D Biol Crystallogr. 2015 Dec 1;71(Pt 12):2494-504. doi:, 10.1107/S1399004715019392. Epub 2015 Nov 27. PMID:26627656<ref>PMID:26627656</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4y9w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Candida parapsilosis]] | [[Category: Candida parapsilosis]] | ||
| - | [[Category: Brynda | + | [[Category: Large Structures]] |
| - | [[Category: Dostal | + | [[Category: Synthetic construct]] |
| - | [[Category: Hruskova-Heidingsfeldova | + | [[Category: Brynda J]] |
| - | [[Category: Mareckova | + | [[Category: Dostal J]] |
| - | [[Category: Pichova | + | [[Category: Hruskova-Heidingsfeldova O]] |
| - | [[Category: Rezacova | + | [[Category: Mareckova L]] |
| - | + | [[Category: Pichova I]] | |
| - | + | [[Category: Rezacova P]] | |
| - | + | ||
Current revision
Aspartic Proteinase Sapp2 Secreted from Candida Parapsilosis at 0.82 A Resolution.
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