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5t3j

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==Histidinol Phosphate Phosphatase(HPP) soaked with selenourea for 10 min==
==Histidinol Phosphate Phosphatase(HPP) soaked with selenourea for 10 min==
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<StructureSection load='5t3j' size='340' side='right' caption='[[5t3j]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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<StructureSection load='5t3j' size='340' side='right'caption='[[5t3j]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5t3j]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T3J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5t3j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T3J FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SEY:SELENOUREA'>SEY</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t3j OCA], [http://pdbe.org/5t3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t3j RCSB], [http://www.ebi.ac.uk/pdbsum/5t3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t3j ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SEY:SELENOUREA'>SEY</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t3j OCA], [https://pdbe.org/5t3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t3j RCSB], [https://www.ebi.ac.uk/pdbsum/5t3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t3j ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/G7J7Q5_MEDTR G7J7Q5_MEDTR]
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Majority of novel X-ray crystal structures of proteins are currently solved using the anomalous diffraction signal provided by selenium after incorporation of selenomethionine instead of natural methionine by genetic engineering methods. However, selenium can be inserted into protein crystals in the form of selenourea (SeC(NH2)2), by adding the crystalline powder of selenourea into mother liquor or cryo-solution with native crystals, in analogy to the classic procedure of heavy-atom derivatization. Selenourea is able to bind to reactive groups at the surface of macromolecules primarily through hydrogen bonds, where the selenium atom may serve as acceptor and amide groups as donors. Selenourea has different chemical properties than heavy-atom reagents and halide ions and provides a convenient way of phasing crystal structures of macromolecules.
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Selenourea: a convenient phasing vehicle for macromolecular X-ray crystal structures.,Luo Z Sci Rep. 2016 Nov 14;6:37123. doi: 10.1038/srep37123. PMID:27841370<ref>PMID:27841370</ref>
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==See Also==
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*[[Inositol monophosphatase 3D structures|Inositol monophosphatase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5t3j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Dauter, Z]]
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[[Category: Large Structures]]
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[[Category: Luo, Z]]
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[[Category: Medicago truncatula]]
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[[Category: Hydrolase]]
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[[Category: Dauter Z]]
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[[Category: Selenourea]]
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[[Category: Luo Z]]

Current revision

Histidinol Phosphate Phosphatase(HPP) soaked with selenourea for 10 min

PDB ID 5t3j

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