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| | ==Crystal structure of a fragment containing the acyltransferase domain of Pks13 from Mycobacterium tuberculosis in the orthorhombic apoform at 2.6 A== | | ==Crystal structure of a fragment containing the acyltransferase domain of Pks13 from Mycobacterium tuberculosis in the orthorhombic apoform at 2.6 A== |
| - | <StructureSection load='3tzw' size='340' side='right' caption='[[3tzw]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='3tzw' size='340' side='right'caption='[[3tzw]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tzw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TZW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tzw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TZW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tzx|3tzx]], [[3tzy|3tzy]], [[3tzz|3tzz]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv3800c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzw OCA], [https://pdbe.org/3tzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tzw RCSB], [https://www.ebi.ac.uk/pdbsum/3tzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzw ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzw OCA], [http://pdbe.org/3tzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tzw RCSB], [http://www.ebi.ac.uk/pdbsum/3tzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O53579_MYCTO O53579_MYCTO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| - | [[Category: Bergeret, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Bon, C]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Mourey, L]] | + | [[Category: Bergeret F]] |
| - | [[Category: Pedelacq, J D]] | + | [[Category: Bon C]] |
| - | [[Category: Acyl carrier protein]] | + | [[Category: Mourey L]] |
| - | [[Category: Acyltransferase]] | + | [[Category: Pedelacq JD]] |
| - | [[Category: Long fatty acid chain transferase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
O53579_MYCTO
Publication Abstract from PubMed
Pks13 is a type I polyketide synthase involved in the final biosynthesis step of mycolic acids, virulence factors, and essential components of the Mycobacterium tuberculosis envelope. Here, we report the biochemical and structural characterization of a 52-kDa fragment containing the acyltransferase domain of Pks13. This fragment retains the ability to load atypical extender units, unusually long chain acyl-CoA with a predilection for carboxylated substrates. High resolution crystal structures were determined for the apo, palmitoylated, and carboxypalmitoylated forms. Structural conservation with type I polyketide synthases and related fatty-acid synthases also extends to the interdomain connections. Subtle changes could be identified both in the active site and in the upstream and downstream linkers in line with the organization displayed by this singular polyketide synthase. More importantly, the crystallographic analysis illustrated for the first time how a long saturated chain can fit in the core structure of an acyltransferase domain through a dedicated channel. The structures also revealed the unexpected binding of a 12-mer peptide that might provide insight into domain-domain interaction.
Biochemical and structural study of the atypical acyltransferase domain from the mycobacterial polyketide synthase pks13.,Bergeret F, Gavalda S, Chalut C, Malaga W, Quemard A, Pedelacq JD, Daffe M, Guilhot C, Mourey L, Bon C J Biol Chem. 2012 Sep 28;287(40):33675-90. Epub 2012 Jul 23. PMID:22825853[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bergeret F, Gavalda S, Chalut C, Malaga W, Quemard A, Pedelacq JD, Daffe M, Guilhot C, Mourey L, Bon C. Biochemical and structural study of the atypical acyltransferase domain from the mycobacterial polyketide synthase pks13. J Biol Chem. 2012 Sep 28;287(40):33675-90. Epub 2012 Jul 23. PMID:22825853 doi:http://dx.doi.org/10.1074/jbc.M111.325639
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