1qo5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:50, 13 December 2023) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qo5.gif|left|200px]]
 
-
{{Structure
+
==Fructose 1,6-bisphosphate Aldolase from Human Liver Tissue==
-
|PDB= 1qo5 |SIZE=350|CAPTION= <scene name='initialview01'>1qo5</scene>, resolution 2.5&Aring;
+
<StructureSection load='1qo5' size='340' side='right'caption='[[1qo5]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=SBA:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBA</scene>, <scene name='pdbsite=SBB:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBB</scene>, <scene name='pdbsite=SBC:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBC</scene>, <scene name='pdbsite=SBD:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBD</scene>, <scene name='pdbsite=SBE:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBE</scene>, <scene name='pdbsite=SBF:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBF</scene>, <scene name='pdbsite=SBG:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBG</scene>, <scene name='pdbsite=SBH:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBH</scene>, <scene name='pdbsite=SBI:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBI</scene>, <scene name='pdbsite=SBJ:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBJ</scene>, <scene name='pdbsite=SBK:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBK</scene>, <scene name='pdbsite=SBL:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBL</scene>, <scene name='pdbsite=SBM:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBM</scene>, <scene name='pdbsite=SBN:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBN</scene>, <scene name='pdbsite=SBO:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBO</scene>, <scene name='pdbsite=SBP:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBP</scene>, <scene name='pdbsite=SBQ:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBQ</scene> and <scene name='pdbsite=SBR:Active+Site+w.+ARG+55+C-1-Phosphate+Group+Of+Substrate+L+...'>SBR</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
<table><tr><td colspan='2'>[[1qo5]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QO5 FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qo5 OCA], [https://pdbe.org/1qo5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qo5 RCSB], [https://www.ebi.ac.uk/pdbsum/1qo5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qo5 ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qo5 OCA], [http://www.ebi.ac.uk/pdbsum/1qo5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qo5 RCSB]</span>
+
== Disease ==
-
}}
+
[https://www.uniprot.org/uniprot/ALDOB_HUMAN ALDOB_HUMAN] Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:[https://omim.org/entry/229600 229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.<ref>PMID:10970798</ref> <ref>PMID:3383242</ref> <ref>PMID:1967768</ref> <ref>PMID:8299883</ref> <ref>PMID:8162030</ref> <ref>PMID:2336380</ref> <ref>PMID:10024431</ref> <ref>PMID:12205126</ref> <ref>PMID:15532022</ref> <ref>PMID:15880727</ref>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ALDOB_HUMAN ALDOB_HUMAN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/1qo5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qo5 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The X-ray crystallographic structure of the human liver isozyme of fructose-1,6-bisphosphate aldolase has been determined by molecular replacement using a tetramer of the human muscle isozyme as a search model. The liver aldolase (B isozyme) crystallized in space group C2, with unit-cell parameters a = 291.1, b = 489.8, c = 103.4 A, alpha = 90, beta = 103.6, gamma = 90 degrees. These large unit-cell parameters result from the presence of 18 subunits in the asymmetric unit: four catalytic tetramers and a dimer from a fifth tetramer positioned on the twofold crystallographic axis. This structure provides further insight into the factors affecting isozyme specificity. It reveals small differences in secondary structure that occur in regions previously determined to be isozyme specific. Two of these regions are at the solvent-exposed enzyme surface away from the active site of the enzyme. The most significant changes are in the flexible C-terminal region of the enzyme, where there is an insertion of an extra alpha-helix. Point mutations of the human liver aldolase are responsible for the disease hereditary fructose intolerance. Sequence information is projected onto the new crystal structure in order to indicate how these mutations bring about reduced enzyme activity and affect structural stability.
-
'''FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE FROM HUMAN LIVER TISSUE'''
+
The structure of human liver fructose-1,6-bisphosphate aldolase.,Dalby AR, Tolan DR, Littlechild JA Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1526-33. Epub 2001, Oct 25. PMID:11679716<ref>PMID:11679716</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1qo5" style="background-color:#fffaf0;"></div>
-
==About this Structure==
+
==See Also==
-
1QO5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QO5 OCA].
+
*[[Aldolase 3D structures|Aldolase 3D structures]]
-
[[Category: Fructose-bisphosphate aldolase]]
+
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Dalby, A R.]]
+
[[Category: Dalby AR]]
-
[[Category: Littlechild, J A.]]
+
[[Category: Littlechild JA]]
-
[[Category: aldolase]]
+
-
[[Category: glycolytic enzyme]]
+
-
[[Category: tim barrel]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:16:37 2008''
+

Current revision

Fructose 1,6-bisphosphate Aldolase from Human Liver Tissue

PDB ID 1qo5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qo5"
Personal tools