4a7p
From Proteopedia
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==Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea== | ==Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea== | ||
| - | <StructureSection load='4a7p' size='340' side='right' caption='[[4a7p]], [[Resolution|resolution]] 3.40Å' scene=''> | + | <StructureSection load='4a7p' size='340' side='right'caption='[[4a7p]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4a7p]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[4a7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_elodea Sphingomonas elodea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A7P FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.403Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7p OCA], [https://pdbe.org/4a7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a7p RCSB], [https://www.ebi.ac.uk/pdbsum/4a7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a7p ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A4UTT2_SPHEL A4UTT2_SPHEL] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Gellan gum, a commercial gelling agent produced by Sphingomonas elodea ATCC 31461, is a high-value microbial exopolysaccharide. UDP-glucose dehydrogenase (UGD; EC 1.1.1.22) is responsible for the NAD-dependent twofold oxidation of UDP-glucose to UDP-glucuronic acid, one of the key components for gellan biosynthesis. S. elodea ATCC 31461 UGD, termed UgdG, was cloned, expressed, purified and crystallized in native and SeMet-derivatized forms in hexagonal and tetragonal space groups, respectively; the crystals diffracted X-rays to 2.40 and 3.40 A resolution, respectively. Experimental phases were obtained for the tetragonal SeMet-derivatized crystal form by a single-wavelength anomalous dispersion experiment. This structure was successfully used as a molecular-replacement probe for the hexagonal crystal form of the native protein. | Gellan gum, a commercial gelling agent produced by Sphingomonas elodea ATCC 31461, is a high-value microbial exopolysaccharide. UDP-glucose dehydrogenase (UGD; EC 1.1.1.22) is responsible for the NAD-dependent twofold oxidation of UDP-glucose to UDP-glucuronic acid, one of the key components for gellan biosynthesis. S. elodea ATCC 31461 UGD, termed UgdG, was cloned, expressed, purified and crystallized in native and SeMet-derivatized forms in hexagonal and tetragonal space groups, respectively; the crystals diffracted X-rays to 2.40 and 3.40 A resolution, respectively. Experimental phases were obtained for the tetragonal SeMet-derivatized crystal form by a single-wavelength anomalous dispersion experiment. This structure was successfully used as a molecular-replacement probe for the hexagonal crystal form of the native protein. | ||
| - | Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461.,Rocha J, Granja AT, Sa-Correia I, Fialho A, Frazao C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt, 1):69-72. Epub 2009 Dec 25. PMID: | + | Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461.,Rocha J, Granja AT, Sa-Correia I, Fialho A, Frazao C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt, 1):69-72. Epub 2009 Dec 25. PMID:020057075<ref>PMID:020057075</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Sphingomonas elodea]] |
| - | [[Category: Fialho | + | [[Category: Fialho AM]] |
| - | [[Category: Frazao | + | [[Category: Frazao C]] |
| - | [[Category: Granja | + | [[Category: Granja AT]] |
| - | [[Category: Rocha | + | [[Category: Rocha J]] |
| - | [[Category: Sa-Correia | + | [[Category: Sa-Correia I]] |
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Current revision
Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea
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