3tx4

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==Crystal Structure of Mutant (C354A) M. tuberculosis LD-transpeptidase type 2==
==Crystal Structure of Mutant (C354A) M. tuberculosis LD-transpeptidase type 2==
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<StructureSection load='3tx4' size='340' side='right' caption='[[3tx4]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
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<StructureSection load='3tx4' size='340' side='right'caption='[[3tx4]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TX4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tx4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TX4 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tur|3tur]], [[3vae|3vae]], [[3u1q|3u1q]], [[3u1p|3u1p]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lppS, MT2594, Rv2518c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tx4 OCA], [https://pdbe.org/3tx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tx4 RCSB], [https://www.ebi.ac.uk/pdbsum/3tx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tx4 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tx4 OCA], [http://pdbe.org/3tx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tx4 RCSB], [http://www.ebi.ac.uk/pdbsum/3tx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tx4 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/LDT2_MYCTO LDT2_MYCTO] Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. Is essential for virulence in a mouse model of acute infection.<ref>PMID:20305661</ref>
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With multidrug-resistant cases of tuberculosis increasing globally, better antibiotic drugs and novel drug targets are becoming an urgent need. Traditional beta-lactam antibiotics that inhibit D,D-transpeptidases are not effective against mycobacteria, in part because mycobacteria rely mostly on L,D-transpeptidases for biosynthesis and maintenance of their peptidoglycan layer. This reliance plays a major role in drug resistance and persistence of Mycobacterium tuberculosis (Mtb) infections. The crystal structure at 1.7 A resolution of the Mtb L,D-transpeptidase Ldt(Mt2) containing a bound peptidoglycan fragment, reported here, provides information about catalytic site organization as well as substrate recognition by the enzyme. Based on our structural, kinetic, and calorimetric data, we propose a catalytic mechanism for Ldt(Mt2) in which both acyl-acceptor and acyl-donor substrates reach the catalytic site from the same, rather than different, entrances. Together, this information provides vital insights to facilitate development of drugs targeting this validated yet unexploited enzyme.
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Targeting the Cell Wall of Mycobacterium tuberculosis: Structure and Mechanism of L,D-Transpeptidase 2.,Erdemli SB, Gupta R, Bishai WR, Lamichhane G, Amzel LM, Bianchet MA Structure. 2012 Dec 5;20(12):2103-15. doi: 10.1016/j.str.2012.09.016. Epub 2012, Oct 25. PMID:23103390<ref>PMID:23103390</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3tx4" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Amzel, L M]]
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[[Category: Large Structures]]
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[[Category: Bianchet, M A]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Erdemli, S]]
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[[Category: Amzel LM]]
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[[Category: Gupta, R]]
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[[Category: Bianchet MA]]
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[[Category: Lamichhane, G]]
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[[Category: Erdemli S]]
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[[Category: Peptidoglycan binding protein]]
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[[Category: Gupta R]]
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[[Category: Protein-peptidoglycan complex]]
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[[Category: Lamichhane G]]

Current revision

Crystal Structure of Mutant (C354A) M. tuberculosis LD-transpeptidase type 2

PDB ID 3tx4

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