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| | ==Crystal structure of Lactobacillus reuteri 121 N-terminally truncated glucansucrase GTFA== | | ==Crystal structure of Lactobacillus reuteri 121 N-terminally truncated glucansucrase GTFA== |
| - | <StructureSection load='4amc' size='340' side='right' caption='[[4amc]], [[Resolution|resolution]] 3.60Å' scene=''> | + | <StructureSection load='4amc' size='340' side='right'caption='[[4amc]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4amc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_23272 Atcc 23272]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AMC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4amc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limosilactobacillus_reuteri Limosilactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AMC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4amc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4amc OCA], [http://pdbe.org/4amc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4amc RCSB], [http://www.ebi.ac.uk/pdbsum/4amc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4amc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4amc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4amc OCA], [https://pdbe.org/4amc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4amc RCSB], [https://www.ebi.ac.uk/pdbsum/4amc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4amc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5SBL9_LIMRT Q5SBL9_LIMRT] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.[ARBA:ARBA00003243] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 23272]] | + | [[Category: Large Structures]] |
| - | [[Category: Dextransucrase]] | + | [[Category: Limosilactobacillus reuteri]] |
| - | [[Category: Dijkhuizen, L]] | + | [[Category: Dijkhuizen L]] |
| - | [[Category: Dijkstra, B W]] | + | [[Category: Dijkstra BW]] |
| - | [[Category: Kralj, S]] | + | [[Category: Kralj S]] |
| - | [[Category: Pijning, T]] | + | [[Category: Pijning T]] |
| - | [[Category: Vujicic-Zagar, A]] | + | [[Category: Vujicic-Zagar A]] |
| - | [[Category: Glycosyltransferase]]
| + | |
| - | [[Category: Reuteransucrase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q5SBL9_LIMRT Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.[ARBA:ARBA00003243]
Publication Abstract from PubMed
The reuteransucrase GTFA from Lactobacillus reuteri 121, which belongs to glycosyl hydrolase family GH70, synthesizes branched alpha-glucans with both alpha-1,6- and alpha-1,4-glycosidic linkages (reuteran) from sucrose. The crystal structure of GTFA-DeltaN, a 118 kDa fragment of GTFA comprising residues 745-1763 and including the catalytic domain, was determined at 3.6 A resolution by molecular replacement. The crystals have large solvent channels and an unusually high solvent content of 85%. GTFA-DeltaN has the same domain arrangement and domain topologies as observed in previously determined GH70 glucansucrase structures. The architecture of the GTFA-DeltaN active site and binding pocket confirms that glucansucrases have a conserved substrate specificity for sucrose. However, this first crystal structure of an alpha-1,6/alpha-1,4-specific glucansucrase shows that residues from conserved sequence motif IV (1128-1136 in GTFA-DeltaN) contribute to the acceptor-binding subsites and that they display differences compared with other structurally characterized glucansucrases. In particular, the structure clarifies the importance of residues following the transition-state stabilizer for product specificity, and especially residue Asn1134, which is in a position to interact with sugar units in acceptor subsite +2.
Structure of the alpha-1,6/alpha-1,4-specific glucansucrase GTFA from Lactobacillus reuteri 121.,Pijning T, Vujicic-Zagar A, Kralj S, Dijkhuizen L, Dijkstra BW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1448-54., doi: 10.1107/S1744309112044168. Epub 2012 Nov 14. PMID:23192022[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pijning T, Vujicic-Zagar A, Kralj S, Dijkhuizen L, Dijkstra BW. Structure of the alpha-1,6/alpha-1,4-specific glucansucrase GTFA from Lactobacillus reuteri 121. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1448-54., doi: 10.1107/S1744309112044168. Epub 2012 Nov 14. PMID:23192022 doi:http://dx.doi.org/10.1107/S1744309112044168
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