4dfb

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==Crystal structure of aminoglycoside phosphotransferase aph(2")-id/aph(2")-iva in complex with kanamycin==
==Crystal structure of aminoglycoside phosphotransferase aph(2")-id/aph(2")-iva in complex with kanamycin==
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<StructureSection load='4dfb' size='340' side='right' caption='[[4dfb]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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<StructureSection load='4dfb' size='340' side='right'caption='[[4dfb]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4dfb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptococcus_casseliflavus"_vaughan_et_al._1979 "streptococcus casseliflavus" vaughan et al. 1979]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3r81 3r81]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DFB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DFB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4dfb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_casseliflavus Enterococcus casseliflavus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3r81 3r81]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DFB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DFB FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KAN:KANAMYCIN+A'>KAN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KAN:KANAMYCIN+A'>KAN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dbx|4dbx]], [[4de4|4de4]], [[4dfu|4dfu]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dfb OCA], [https://pdbe.org/4dfb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dfb RCSB], [https://www.ebi.ac.uk/pdbsum/4dfb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dfb ProSAT]</span></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aph(2")-Id ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37734 "Streptococcus casseliflavus" Vaughan et al. 1979])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dfb OCA], [http://pdbe.org/4dfb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dfb RCSB], [http://www.ebi.ac.uk/pdbsum/4dfb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dfb ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/O68183_ENTCA O68183_ENTCA]
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Kinase-mediated resistance to antibiotics is a significant clinical challenge. These enzymes share a common protein fold characteristic of Ser/Thr/Tyr protein kinases. We screened 14 antibiotic resistance kinases against 80 chemically diverse protein kinase inhibitors to map resistance kinase chemical space. The screens identified molecules with both broad and narrow inhibition profiles, proving that protein kinase inhibitors offer privileged chemical matter with the potential to block antibiotic resistance. One example is the flavonol quercetin, which inhibited a number of resistance kinases in vitro and in vivo. This activity was rationalized by determination of the crystal structure of the aminoglycoside kinase APH(2'')-IVa in complex with quercetin and its antibiotic substrate kanamycin. Our data demonstrate that protein kinase inhibitors offer chemical scaffolds that can block antibiotic resistance, providing leads for co-drug design.
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A small molecule discrimination map of the antibiotic resistance kinome.,Shakya T, Stogios PJ, Waglechner N, Evdokimova E, Ejim L, Blanchard JE, McArthur AG, Savchenko A, Wright GD Chem Biol. 2011 Dec 23;18(12):1591-601. PMID:22195561<ref>PMID:22195561</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4dfb" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Phosphotransferase|Phosphotransferase]]
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*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Streptococcus casseliflavus vaughan et al. 1979]]
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[[Category: Enterococcus casseliflavus]]
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[[Category: Anderson, W F]]
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[[Category: Large Structures]]
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[[Category: Structural genomic]]
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[[Category: Anderson WF]]
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[[Category: Egorova, E]]
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[[Category: Di leo R]]
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[[Category: Evdokimova, E]]
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[[Category: Egorova E]]
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[[Category: Li, H]]
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[[Category: Evdokimova E]]
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[[Category: Minasov, G]]
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[[Category: Li H]]
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[[Category: Osipiuk, J]]
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[[Category: Minasov G]]
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[[Category: Savchenko, A]]
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[[Category: Osipiuk J]]
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[[Category: Stogios, P J]]
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[[Category: Savchenko A]]
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[[Category: Leo, R Di]]
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[[Category: Stogios PJ]]
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[[Category: Aminoglycoside phosphotransferase]]
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[[Category: Aminoglycoside]]
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[[Category: Antibiotic]]
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[[Category: Csgid]]
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[[Category: Eukaryotic protein kinase-like fold]]
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[[Category: Kanamycin]]
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[[Category: Kinase]]
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[[Category: Transferase]]
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[[Category: Transferase-antibiotic complex]]
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Current revision

Crystal structure of aminoglycoside phosphotransferase aph(2")-id/aph(2")-iva in complex with kanamycin

PDB ID 4dfb

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