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| | ==Crystal structure of the Hy19.3 type II NKT TCR== | | ==Crystal structure of the Hy19.3 type II NKT TCR== |
| - | <StructureSection load='4elk' size='340' side='right' caption='[[4elk]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='4elk' size='340' side='right'caption='[[4elk]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4elk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ELK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4elk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ELK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4elm|4elm]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Valpha1 (mouse variable domain, human constant domain) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Vbeta16 (mouse variable domain, human constant domain) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4elk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elk OCA], [https://pdbe.org/4elk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4elk RCSB], [https://www.ebi.ac.uk/pdbsum/4elk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4elk ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4elk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elk OCA], [http://pdbe.org/4elk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4elk RCSB], [http://www.ebi.ac.uk/pdbsum/4elk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4elk ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Girardi, E]] | + | [[Category: Mus musculus]] |
| - | [[Category: Iyer, P]] | + | [[Category: Girardi E]] |
| - | [[Category: Kumar, V]] | + | [[Category: Iyer P]] |
| - | [[Category: Mac, T T]] | + | [[Category: Kumar V]] |
| - | [[Category: Maricic, I]] | + | [[Category: Mac TT]] |
| - | [[Category: Wang, J]] | + | [[Category: Maricic I]] |
| - | [[Category: Zajonc, D M]] | + | [[Category: Wang J]] |
| - | [[Category: Antigen presentation]]
| + | [[Category: Zajonc DM]] |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Type ii nkt cell]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Glycolipids presented by the major histocompatibility complex (MHC) class I homolog CD1d are recognized by natural killer T cells (NKT cells) characterized by either a semi-invariant T cell antigen receptor (TCR) repertoire (type I NKT cells or iNKT cells) or a relatively variable TCR repertoire (type II NKT cells). Here we describe the structure of a type II NKT cell TCR in complex with CD1d-lysosulfatide. Both TCR alpha-chains and TCR beta-chains made contact with the CD1d molecule with a diagonal footprint, typical of MHC-TCR interactions, whereas the antigen was recognized exclusively with a single TCR chain, similar to the iNKT cell TCR. Type II NKT cell TCRs, therefore, recognize CD1d-sulfatide complexes by a distinct recognition mechanism characterized by the TCR-binding features of both iNKT cells and conventional peptide-reactive T cells.
Type II natural killer T cells use features of both innate-like and conventional T cells to recognize sulfatide self antigens.,Girardi E, Maricic I, Wang J, Mac TT, Iyer P, Kumar V, Zajonc DM Nat Immunol. 2012 Sep;13(9):851-6. doi: 10.1038/ni.2371. Epub 2012 Jul 22. PMID:22820602[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Girardi E, Maricic I, Wang J, Mac TT, Iyer P, Kumar V, Zajonc DM. Type II natural killer T cells use features of both innate-like and conventional T cells to recognize sulfatide self antigens. Nat Immunol. 2012 Sep;13(9):851-6. doi: 10.1038/ni.2371. Epub 2012 Jul 22. PMID:22820602 doi:http://dx.doi.org/10.1038/ni.2371
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