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| | ==Tic22 from Plasmodium falciparum== | | ==Tic22 from Plasmodium falciparum== |
| - | <StructureSection load='4e6z' size='340' side='right' caption='[[4e6z]], [[Resolution|resolution]] 2.15Å' scene=''> | + | <StructureSection load='4e6z' size='340' side='right'caption='[[4e6z]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4e6z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4e6z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E6Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1501Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFE1460w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6z OCA], [https://pdbe.org/4e6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e6z RCSB], [https://www.ebi.ac.uk/pdbsum/4e6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6z ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6z OCA], [http://pdbe.org/4e6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e6z RCSB], [http://www.ebi.ac.uk/pdbsum/4e6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6z ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8I3H4_PLAF7 Q8I3H4_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plaf7]] | + | [[Category: Large Structures]] |
| - | [[Category: Glaser, S]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Higgins, M K]] | + | [[Category: Glaser S]] |
| - | [[Category: Apicoplast]] | + | [[Category: Higgins MK]] |
| - | [[Category: Import protein]]
| + | |
| - | [[Category: Tic complex]]
| + | |
| - | [[Category: Transport protein]]
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| Structural highlights
Function
Q8I3H4_PLAF7
Publication Abstract from PubMed
Most plastids proteins are post-translationally imported into organelles through multisubunit translocons. The TIC and TOC complexes perform this role in the two membranes of the plant chloroplast and in the inner two membranes of the apicoplasts of the apicomplexan parasites, T. gondii and P. falciparum. Tic22 is a ubiquitous intermembrane translocon component that interacts with translocating proteins. Here, we demonstrate that T. gondii Tic22 is an apicoplast-localized protein, essential for parasite survival and protein import into the apicoplast stroma. The structure of Tic22 from P. falciparum reveals a fold conserved from cyanobacteria to plants, which displays a non-polar groove on each sides of the molecule. We show that these grooves allow Tic22 to act as a chaperone. General chaperones are common components of protein translocation systems where they maintain cargo proteins in an unfolded conformation during transit. Such a chaperone had not been identified in the intermembrane space of plastids and we propose that Tic22 fulfils this role.
Tic22 is an essential chaperone required for protein import into the apicoplast.,Glaser S, van Dooren GG, Agrawal S, Brooks CF, McFadden GI, Striepen B, Higgins MK J Biol Chem. 2012 Oct 1. PMID:23027875[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Glaser S, van Dooren GG, Agrawal S, Brooks CF, McFadden GI, Striepen B, Higgins MK. Tic22 is an essential chaperone required for protein import into the apicoplast. J Biol Chem. 2012 Oct 1. PMID:23027875 doi:http://dx.doi.org/10.1074/jbc.M112.405100
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