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| | ==The Crystal Structure of the Neisserial Esterase D.== | | ==The Crystal Structure of the Neisserial Esterase D.== |
| - | <StructureSection load='4b6g' size='340' side='right' caption='[[4b6g]], [[Resolution|resolution]] 1.40Å' scene=''> | + | <StructureSection load='4b6g' size='340' side='right'caption='[[4b6g]], [[Resolution|resolution]] 1.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4b6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neimb Neimb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B6G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4b6g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_MC58 Neisseria meningitidis MC58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B6G FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-formylglutathione_hydrolase S-formylglutathione hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.12 3.1.2.12] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b6g OCA], [http://pdbe.org/4b6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b6g RCSB], [http://www.ebi.ac.uk/pdbsum/4b6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b6g ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b6g OCA], [https://pdbe.org/4b6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b6g RCSB], [https://www.ebi.ac.uk/pdbsum/4b6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b6g ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9JZ43_NEIMB Q9JZ43_NEIMB] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Neimb]] | + | [[Category: Large Structures]] |
| - | [[Category: S-formylglutathione hydrolase]] | + | [[Category: Neisseria meningitidis MC58]] |
| - | [[Category: Counago, R M]] | + | [[Category: Counago RM]] |
| - | [[Category: Kobe, B]] | + | [[Category: Kobe B]] |
| - | [[Category: Alpha/beta serine hydrolase]]
| + | |
| - | [[Category: Formaldehyde detoxification]]
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| - | [[Category: Hydrolase]]
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| Structural highlights
Function
Q9JZ43_NEIMB
Publication Abstract from PubMed
<b>Aim:</b> The glutathione-dependent, AdhC-EstD formaldehyde detoxification system is found in eukaryotes and prokaryotes. It is established that it confers protection against formaldehyde that is produced from environmental sources or methanol metabolism. Thus, its presence in the human host adapted bacterial pathogen <i>Neisseria meningitidis</i> is intriguing. This work defined the biological function of this system in the meningococcus using phenotypic analyses of mutants linked to biochemical and structural characterisation of purified enzymes. <b>Results:</b> We demonstrated that mutants in the <i>adhC</i> and/or <i>estD</i> were sensitive to killing by formaldehyde. Inactivation of <i>adhC</i> and/or <i>estD</i> also led to a loss of viability in biofilm communities, even in the absence of exogenous formaldehyde. Detailed biochemical and structural analyses of the esterase component demonstrated that <i>S</i>-formylglutathione was the only biologically relevant substrate for EstD. We further showed that an absolutely conserved cysteine residue was covalently modified by <i>S</i>-glutathionylation. This leads to inactivation of EstD. <b>Innovation:</b> The results provide several conceptual innovations. They provide new insight into formaldehyde detoxification in bacteria that do not generate formaldehyde during the catabolism of methanol. Our results also indicate that the conserved cysteine, found in all EstD enzymes from humans to microbes, is a site of enzyme regulation, probably via <i>S</i>-glutathionylation. <b>Conclusion:</b> The <i>adhC/estD</i> system protects against formaldehyde produced during endogenous metabolism.
A glutathione-dependent detoxification system is required for formaldehyde resistance and optimal survival of <i>Neisseria meningitidis</i> in biofilms.,Chen NH, Counago RM, Djoko KY, Jennings MP, Apicella MA, Kobe B, McEwan AG Antioxid Redox Signal. 2012 Sep 3. PMID:22937752[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chen NH, Counago RM, Djoko KY, Jennings MP, Apicella MA, Kobe B, McEwan AG. A glutathione-dependent detoxification system is required for formaldehyde resistance and optimal survival of Neisseria meningitidis in biofilms. Antioxid Redox Signal. 2012 Sep 3. PMID:22937752 doi:http://dx.doi.org/10.1089/ars.2012.4749
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