|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of the Novel Phenazine Prenyltransferase EpzP (wildtype)== | | ==Crystal structure of the Novel Phenazine Prenyltransferase EpzP (wildtype)== |
| - | <StructureSection load='4ee8' size='340' side='right' caption='[[4ee8]], [[Resolution|resolution]] 1.93Å' scene=''> | + | <StructureSection load='4ee8' size='340' side='right'caption='[[4ee8]], [[Resolution|resolution]] 1.93Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ee8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1619 As 4.1619]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EE8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ee8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cinnamonensis Streptomyces cinnamonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EE8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ee6|4ee6]], [[4ee7|4ee7]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epzP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1900 AS 4.1619])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ee8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee8 OCA], [https://pdbe.org/4ee8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ee8 RCSB], [https://www.ebi.ac.uk/pdbsum/4ee8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee8 OCA], [http://pdbe.org/4ee8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ee8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/E5KWG9_STRCM E5KWG9_STRCM] |
| - | Prenyltransferases (PTs) catalyze the regioselective transfer of prenyl moieties onto aromatic substrates in biosynthetic pathways of microbial secondary metabolites. Therefore, these enzymes contribute to the chemical diversity of natural products. Prenylation is frequently essential for the pharmacological properties of these metabolites, including their antibiotic and antitumor activities. Recently, the first phenazine PTs, termed EpzP and PpzP, were isolated and biochemically characterized. The two enzymes play a central role in the biosynthesis of endophenazines by catalyzing the regiospecific prenylation of 5,10-dihydrophenazine-1-carboxylic acid (dhPCA) in the secondary metabolism of two different Streptomyces strains. Here we report crystal structures of EpzP in its unliganded state as well as bound to S-thiolodiphosphate (SPP), thus defining the first three-dimensional structures for any phenazine PT. A model of a ternary complex resulted from in silico modeling of dhPCA and site-directed mutagenesis. The structural analysis provides detailed insight into the likely mechanism of phenazine prenylation. The catalytic mechanism suggested by the structure identifies amino acids that are required for catalysis. Inspection of the structures and the model of the ternary complex furthermore allowed us to rationally engineer EpzP variants with up to 14-fold higher catalytic reaction rate compared to the wild-type enzyme. This study therefore provides a solid foundation for additional enzyme modifications that should result in efficient, tailor-made biocatalysts for phenazines production.
| + | |
| - | | + | |
| - | Structure-based engineering increased the catalytic turnover rate of a novel phenazine prenyltransferase.,Zocher G, Saleh O, Heim JB, Herbst DA, Heide L, Stehle T PLoS One. 2012;7(10):e48427. doi: 10.1371/journal.pone.0048427. Epub 2012 Oct 31. PMID:23119011<ref>PMID:23119011</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4ee8" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: As 4 1619]] | + | [[Category: Large Structures]] |
| - | [[Category: Stehle, T]] | + | [[Category: Streptomyces cinnamonensis]] |
| - | [[Category: Zocher, G]] | + | [[Category: Stehle T]] |
| - | [[Category: Dihydrophenazine carboxylate prenyltransferase]] | + | [[Category: Zocher G]] |
| - | [[Category: Pt fold]]
| + | |
| - | [[Category: Transferase]]
| + | |
